Western blot abreview for Anti-GAPDH antibody [EPR16891]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (ARPE19, hfRPE, HEK293)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
ARPE19, hfRPE, HEK293
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Licor Odyssey Blocking Buffer
Dilution
1/5000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: IRDye 800 CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Licor Odyssey Clx
Negative control
Blank lane (not showed)
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 36 kDa
Positive control
ARPE19, hfRPE, HEK293 cells

Additional data

Additional Notes
PVDF membrane Homogenization buffer: RIPA with mixed phosphatase and protease inhibitors Lane 1: ARPE19 cells Lane 2: hfRPE cells Lane 3: HEK293 cells

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Submitted Oct 25 2016

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