Western blot abreview for Anti-GAPDH antibody [EPR16891]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Fibroblasts)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/5000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 37 kDa

Additional data

PubMed ID
PMID: 27882921: View

Abcam user community

Verified customer

Submitted Nov 29 2016

Sign up