Western blot abreview for Anti-GAPDH antibody [EPR16891]

Good
Abreviews
Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (E. coli)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Specification
E. coli
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/10000

Secondary antibody

Name
Non-Abcam antibody was used: secondary anti-rabbit IgG, HRP linked antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: 37 kDa Non-specific: 35 kDa

Additional data

Abcam response
Thank you for participating in our AbTrial program and your valuable feedback about the use of ab181602 in E. coli and Western Blot. We have not tested the antibody in this species before and we are pleased to see that the antibody is working well for you. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.

Dr. Haibo Wang

Verified customer

Submitted Feb 27 2018

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