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  1. Link

    gapdh-antibody-epr6256-loading-control-ab128915.pdf

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RecombinantRabMAb

Recombinant Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)

  • Datasheet
  • SDS
Reviews (11) Submit a question References (197)

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Western blot - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
  • Western blot - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
  • Western blot - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
  • Flow Cytometry (Intracellular) - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
  • Immunoprecipitation - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
  • Flow Cytometry (Intracellular) - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
  • Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6256] to GAPDH - Loading Control
  • Suitable for: WB, IP, ICC/IF, IHC-P, Flow Cyt (Intra)
  • Reacts with: Human, African green monkey

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 APC Biotin Carrier Free HRP PerCP

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Overview

  • Product name

    Anti-GAPDH antibody [EPR6256] - Loading Control
    See all GAPDH primary antibodies
  • Description

    Rabbit monoclonal [EPR6256] to GAPDH - Loading Control
  • Host species

    Rabbit
  • Specificity

    The African green monkey recommendation is based on the WB results. We do not guarantee IHC-P for African green monkey.

  • Tested applications

    Suitable for: WB, IP, ICC/IF, IHC-P, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Human, African green monkey
  • Immunogen

    Synthetic peptide within Human GAPDH aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P04406

  • Positive control

    • WB: 293T, HeLa, HepG2, HUVEC, MCF7 and SH-SY5Y cell lysates. ICC/IF: HeLa and MCF7 cells. IHC-P: Human pancreas tissue, Human bladder carcinoma. Flow Cyt (intra): HeLa cells. IP: HeLa cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6256
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Huntington's disease
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Isotype/Loading Controls
    • Loading Controls
    • GAPDH
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • HRP Anti-GAPDH antibody [EPR6256] - Loading Control (ab185059)
    • Anti-GAPDH antibody [EPR6256] - BSA and Azide free (ab186930)
    • Biotin Anti-GAPDH antibody [EPR6256] - Loading Control (ab195904)
    • Alexa Fluor® 647 Anti-GAPDH antibody [EPR6256] (ab215227)
    • PerCP Anti-GAPDH antibody [EPR6256] (ab216377)
    • APC Anti-GAPDH antibody [EPR6256] (ab221270)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 nuclear extract lysate (ab14660)
    • MCF7 nuclear extract lysate (ab14860)
    • MCF7 membrane extract lysate (ab29539)
    • HeLa membrane extract lysate (ab29547)
    • HEK-293 whole cell lysate (ab7902)
  • Recombinant Protein

    • Recombinant Mouse GAPDH protein (ab202148)
    • Recombinant Human GAPDH protein (ab77109)
    • Recombinant human GAPDH protein (Active) (ab82633)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (9)
1/10000 - 1/50000. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
IP
1/10 - 1/100.
ICC/IF (1)
1/250 - 1/500.

2.0 µg/ml

IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/250.

The African green monkey recommendation is based on the WB results. We do not guarantee IHC-P for African green monkey.

Flow Cyt (Intra)
1/20.

For unpurified use at 1/100 - 1/1000.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB
1/10000 - 1/50000. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
IP
1/10 - 1/100.
ICC/IF
1/250 - 1/500.

2.0 µg/ml

IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/250.

The African green monkey recommendation is based on the WB results. We do not guarantee IHC-P for African green monkey.

Flow Cyt (Intra)
1/20.

For unpurified use at 1/100 - 1/1000.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • Sequence similarities

    Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • Post-translational
    modifications

    S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Target information above from: UniProt accession P04406 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2597 Human
    • Omim: 138400 Human
    • SwissProt: P04406 Human
    • Unigene: 544577 Human
    • Unigene: 592355 Human
    • Unigene: 598320 Human
    • Alternative names

      • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
      • aging associated gene 9 protein antibody
      • Aging-associated gene 9 protein antibody
      • BARS-38 antibody
      • cb609 antibody
      • EC 1.2.1.12 antibody
      • Epididymis secretory sperm binding protein Li 162eP antibody
      • G3P_HUMAN antibody
      • G3PD antibody
      • G3PDH antibody
      • GAPD antibody
      • GAPDH antibody
      • Glyceraldehyde 3 phosphate dehydrogenase antibody
      • glyceraldehyde 3-PDH antibody
      • Glyceraldehyde-3-phosphate dehydrogenase antibody
      • HEL-S-162eP antibody
      • KNC-NDS6 antibody
      • MGC102544 antibody
      • MGC102546 antibody
      • MGC103190 antibody
      • MGC103191 antibody
      • MGC105239 antibody
      • MGC127711 antibody
      • MGC88685 antibody
      • OCAS, p38 component antibody
      • OCT1 coactivator in S phase, 38-KD component antibody
      • peptidyl cysteine S nitrosylase GAPDH antibody
      • Peptidyl-cysteine S-nitrosylase GAPDH antibody
      • wu:fb33a10 antibody
      see all

    Images

    • Western blot - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
      Western blot - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
      Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915) at 1/20000 dilution (Purified) + COS-1 (African green monkey  kidney fibroblast-like) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 36 kDa
      Observed band size: 36 kDa

    • Western blot - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
      Western blot - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
      Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915) at 1/20000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 36 kDa
      Observed band size: 36 kDa

    • Western blot - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
      Western blot - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
      All lanes : Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915) at 1/10000 dilution (unpurified)

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
      Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
      Lane 4 : HUVEC (human umbilical vein endothelial cell line) cell lysate
      Lane 5 : MCF7 (human breast adenocarcinoma cell line) cell lysate
      Lane 6 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 36 kDa
      Observed band size: 35 kDa why is the actual band size different from the predicted?



      Secondary antibody - anti-rabbit HRP (ab6721)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder carcinoma tissue sections labeling GAPDH with purified ab128915 at 1/2000 dilution (0.06 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
      Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)

      Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GAPDH with purified ab128915 at 1/250 dilution (0.4 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Flow Cytometry (Intracellular) - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
      Flow Cytometry (Intracellular) - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)

      Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GAPDH with purified ab128915 at 1/20 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Immunoprecipitation - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
      Immunoprecipitation - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)

      ab128915 (purified) at 1/20 dilution (0.5ug) immunoprecipitating GAPDH in HeLa whole cell lysates.
      Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates 10ug
      Lane 2 (+): ab128915 & HeLa whole cell lysates
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab128915 in HeLa whole cell lysates
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)

      IHC image of ab128915 (unpurified) staining GAPDH in human pancreas* formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab128915, 1:250 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
      Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)

      ab128915 (unpurified) staining GAPDH in HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab128915 at 2μg/ml and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an goat anti-rabbit AlexaFluor®488 (ab150081) at 2 μg/ml (shown in green) and goat anti-mouse AlexaFluor®594 (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.

      Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

    • Flow Cytometry (Intracellular) - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
      Flow Cytometry (Intracellular) - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)

      Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with ab128915 (unpurified) (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128915, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was goat anti-rabbit Alexa Fluor® 488 (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
      Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)This image is courtesy of an Abreview submitted by Kirk McManus

      ab128915 (unpurified) staining GAPDH in human HeLa (human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counter stained with DAPI.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
      Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)

      ab128915 (unpurified), at 1/250, staining GAPDH in MCF7 (human breast adenocarcinoma cell line) cells by immunofluorescence.

    • Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
      Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (197)

    Publishing research using ab128915? Please let us know so that we can cite the reference in this datasheet.

    ab128915 has been referenced in 197 publications.

    • Lin H  et al. Long Noncoding RNA WEE2-AS1 Plays an Oncogenic Role in Glioblastoma by Functioning as a Molecular Sponge for MicroRNA-520f-3p. Oncol Res 28:591-603 (2021). PubMed: 32838835
    • Fang J  et al. Investigating the effect of lncRNA HOTAIR on apoptosis induced by myocardial ischemia-reperfusion injury. Mol Med Rep 23:N/A (2021). PubMed: 33398378
    • Lu C  et al. Tumor necrosis factor receptor type 1-associated death domain (TRADD) regulates epithelial-mesenchymal transition (EMT), M1/M2 macrophage polarization and ectopic endometrial cysts formation in endometriosis. Ann Transl Med 9:148 (2021). PubMed: 33569450
    • Yun Z  et al. Long non-coding RNA CERS6-AS1 facilitates the oncogenicity of pancreatic ductal adenocarcinoma by regulating the microRNA-15a-5p/FGFR1 axis. Aging (Albany NY) 13:6041-6054 (2021). PubMed: 33581689
    • Chen H  et al. Long Intergenic Non-Coding RNA LINC00885 Promotes Tumorigenesis of Cervical Cancer by Upregulating MACC1 Expression Through Serving as a Competitive Endogenous RNA for microRNA-432-5p. Cancer Manag Res 13:1435-1447 (2021). PubMed: 33603486
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 11 Abreviews or Q&A

    Western blot abreview for Anti-GAPDH antibody [EPR6256]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (hESC, Cancer-various)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    100000 cells
    Specification
    hESC, Cancer-various
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 18 2015

    Western blot abreview for Anti-GAPDH antibody [EPR6256]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Non-reduced Denaturing
    Sample
    Human Cell lysate - whole cell (lung)
    Specification
    lung
    Treatment
    1mM Propanal for 48h
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Ms. Junghee Lim

    Verified customer

    Submitted Jan 13 2015

    Western blot abreview for Anti-GAPDH antibody [EPR6256]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Sample
    Human Cell lysate - whole cell (non small cell lung cancer)
    Specification
    non small cell lung cancer
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Satyendra Tripathi

    Verified customer

    Submitted Sep 25 2014

    Western blot abreview for Anti-GAPDH antibody [EPR6256]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Non-reduced Denaturing (4-12% Nupage gels)
    Sample
    Mouse Tissue lysate - whole (Liver)
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 18 2014

    Western blot abreview for Anti-GAPDH [EPR6256] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    African Green Monkey Cell lysate - whole cell (COS-1 cells)
    Specification
    COS-1 cells
    Treatment
    TGF-beta, 24 hours, 50 pM or untreated
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 07 2014

    Western blot abreview for Anti-GAPDH [EPR6256] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (RAMOS cells)
    Specification
    RAMOS cells
    Treatment
    TGF-beta, 24 hours, 50 pM or untreated
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 07 2014

    Western blot abreview for Anti-GAPDH [EPR6256] antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (NMuMG cells)
    Specification
    NMuMG cells
    Treatment
    TGF-beta, 24 hours, 50 pM or untreated
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 07 2014

    ELISA abreview for Anti-GAPDH [EPR6256] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Recombinant protein (HEK293)
    Specification
    HEK293
    Type
    Sandwich (Detection)
    Blocking step
    SuperBlock PBS Pierce #37580 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10µg/mL · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 14 2014

    Western blot abreview for Anti-GAPDH antibody [EPR6256]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (8)
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic fibroblasts (MEFs))
    Specification
    mouse embryonic fibroblasts (MEFs)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 21 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GAPDH antibody [EPR6256]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde
    Read More

    DR. Kirk Mcmanus

    Verified customer

    Submitted Jan 22 2014

    1-10 of 11 Abreviews or Q&A

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