Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)
- Datasheet
- References (32)
- Protocols
Overview
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Product nameAnti-GAPDH antibody [GA1R] - Loading Control
See all GAPDH primary antibodies -
DescriptionMouse monoclonal [GA1R] to GAPDH - Loading Control
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Host speciesMouse
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Specificityab125247 recognizes native and denatured forms of GAPDH.
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Tested applicationsSuitable for: WB, Dot blot, ELISAmore details
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Species reactivityReacts with: Mouse, Rat, Rabbit, Chicken, Hamster, Human, Saccharomyces cerevisiae, Schizosaccharomyces pombe
Predicted to work with: a wide range of other species
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Immunogen
Recombinant GAPDH.
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Positive control
- Human, Mouse, Rat, Rabbit, Chicken and Hamster tissue lysates; BL-21 bacteria, Sf9 insect and Saccharomyces cerevisiae cell lysates
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General notes
Store at 4°C (add 0.05% NaN3) for several days to weeks.
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityProtein A purified
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Purification notesProtein A affinity chromatography from TCS
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ClonalityMonoclonal
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Clone numberGA1R
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab125247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa). with ECL. | |
| Dot blot | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. |
Target
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FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
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PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
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Sequence similaritiesBelongs to the glyceraldehyde-3-phosphate dehydrogenase family.
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Post-translational
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
- Information by UniProt
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Database links
- Entrez Gene: 374193 Chicken
- Entrez Gene: 2597 Human
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Entrez Gene: 100009074 Rabbit
- Entrez Gene: 24383 Rat
- Entrez Gene: 685186 Rat
- Omim: 138400 Human
see all -
Alternative names
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
Images
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![Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)](https://www.abcam.com/ps/products/125/ab125247/Images/GAPDH-Primary-antibodies-ab125247-1.jpg)
Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)All lanes : Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) at 1/2000 dilution
Lane 1 : Human tissue lysate
Lane 2 : Mouse tissue lysate
Lane 3 : Rat tissue lysate
Lane 4 : Rabbit tissue lysate
Lane 5 : Chicken tissue lysate
Lane 6 : Hamster tissue lysate
Lysates/proteins at 5 µg per lane.
Developed using the ECL technique.
Predicted band size: 36 kDa -
![Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)](https://www.abcam.com/ps/products/125/ab125247/Images/GAPDH-Primary-antibodies-ab125247-2.jpg)
Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)All lanes : Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) at 1/2000 dilution
Lane 1 : BL21 bacterial lysate
Lane 2 : Sf9 insect lysate
Lane 3 : Saccharomyces cerevisiae lysate
Lysates/proteins at 5 µg per lane.
Developed using the ECL technique.
Predicted band size: 36 kDa
Protocols
References
This product has been referenced in:
- Chen S et al. Lentivirus mediated ?-interferon-inducible lysosomal thiol reductase (GILT) knockdown suppresses human glioma U373MG cell proliferation. Biochem Biophys Res Commun 509:182-187 (2019). Read more (PubMed: 30587343) »
- Shiraishi T et al. Mesothelin expression has prognostic value in stage ??/??? colorectal cancer. Virchows Arch 474:297-307 (2019). Read more (PubMed: 30603773) »
