For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    gapdh-antibody-ga1r-loading-control-ab125247.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurology process Neurodegenerative disease Huntington's disease
Share by email

Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)

  • Datasheet
  • SDS
Reviews (6) Submit a question References (70)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)
  • Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)

Key features and details

  • Mouse monoclonal [GA1R] to GAPDH - Loading Control
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Rabbit, Chicken, Hamster, Human, Saccharomyces cerevisiae, Escherichia coli, Spodoptera frugiperda (SF9 cells)
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Mouse GAPDH protein (ab202148)
Assay
Product image
Cathepsin B Activity Assay Kit (Fluorometric) (ab65300)
Primary
Product image
Anti-Chromogranin A antibody [EP1030Y] (ab68271)

View more associated products

Overview

  • Product name

    Anti-GAPDH antibody [GA1R] - Loading Control
    See all GAPDH primary antibodies
  • Description

    Mouse monoclonal [GA1R] to GAPDH - Loading Control
  • Host species

    Mouse
  • Specificity

    ab125247 recognizes native and denatured forms of GAPDH.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Rabbit GAPDH.

  • Positive control

    • Human, Mouse, Rat, Rabbit, Chicken and Hamster tissue lysates; BL-21 bacteria, Sf9 insect and Saccharomyces cerevisiae cell lysates
  • General notes

    Store at 4°C (add 0.05% NaN3) for several days to weeks.

    This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Protein A affinity chromatography from TCS
  • Clonality

    Monoclonal
  • Clone number

    GA1R
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Huntington's disease
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Isotype/Loading Controls
    • Loading Controls
    • GAPDH
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Mouse GAPDH protein (ab202148)
    • Recombinant Human GAPDH protein (ab77109)
    • Recombinant human GAPDH protein (Active) (ab82633)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab125247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
a wide range of other species
Application Abreviews Notes
WB (6)
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa). with ECL.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa). with ECL.

Target

  • Function

    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • Sequence similarities

    Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • Post-translational
    modifications

    S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Target information above from: UniProt accession P04406 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 374193 Chicken
    • Entrez Gene: 2597 Human
    • Entrez Gene: 100042025 Mouse
    • Entrez Gene: 14433 Mouse
    • Entrez Gene: 100009074 Rabbit
    • Entrez Gene: 24383 Rat
    • Entrez Gene: 685186 Rat
    • Omim: 138400 Human
    • SwissProt: P00356 Chicken
    • SwissProt: P04406 Human
    • SwissProt: P16858 Mouse
    • SwissProt: P46406 Rabbit
    • SwissProt: P04797 Rat
    • Unigene: 544577 Human
    • Unigene: 592355 Human
    • Unigene: 598320 Human
    • Unigene: 304088 Mouse
    • Unigene: 309092 Mouse
    • Unigene: 317779 Mouse
    • Unigene: 343110 Mouse
    • Unigene: 392463 Mouse
    • Unigene: 392480 Mouse
    • Unigene: 414470 Mouse
    • Unigene: 458138 Mouse
    • Unigene: 458416 Mouse
    • Unigene: 475698 Mouse
    • Unigene: 129558 Rat
    • Unigene: 91450 Rat
    see all
  • Alternative names

    • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
    • aging associated gene 9 protein antibody
    • Aging-associated gene 9 protein antibody
    • BARS-38 antibody
    • cb609 antibody
    • EC 1.2.1.12 antibody
    • Epididymis secretory sperm binding protein Li 162eP antibody
    • G3P_HUMAN antibody
    • G3PD antibody
    • G3PDH antibody
    • GAPD antibody
    • GAPDH antibody
    • Glyceraldehyde 3 phosphate dehydrogenase antibody
    • glyceraldehyde 3-PDH antibody
    • Glyceraldehyde-3-phosphate dehydrogenase antibody
    • HEL-S-162eP antibody
    • KNC-NDS6 antibody
    • MGC102544 antibody
    • MGC102546 antibody
    • MGC103190 antibody
    • MGC103191 antibody
    • MGC105239 antibody
    • MGC127711 antibody
    • MGC88685 antibody
    • OCAS, p38 component antibody
    • OCT1 coactivator in S phase, 38-KD component antibody
    • peptidyl cysteine S nitrosylase GAPDH antibody
    • Peptidyl-cysteine S-nitrosylase GAPDH antibody
    • wu:fb33a10 antibody
    see all

Images

  • Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)
    Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)
    All lanes : Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) at 1/2000 dilution

    Lane 1 : Human tissue lysate
    Lane 2 : Mouse tissue lysate
    Lane 3 : Rat tissue lysate
    Lane 4 : Rabbit tissue lysate
    Lane 5 : Chicken tissue lysate
    Lane 6 : Hamster tissue lysate

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 36 kDa

  • Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)
    Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)
    All lanes : Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) at 1/2000 dilution

    Lane 1 : BL21 bacterial lysate
    Lane 2 : Sf9 insect lysate
    Lane 3 : Saccharomyces cerevisiae lysate

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 36 kDa

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (70)

Publishing research using ab125247? Please let us know so that we can cite the reference in this datasheet.

ab125247 has been referenced in 70 publications.

  • Boivin M  et al. Reduced autophagy upon C9ORF72 loss synergizes with dipeptide repeat protein toxicity in G4C2 repeat expansion disorders. EMBO J 39:e100574 (2020). PubMed: 31930538
  • Worden EJ  et al. Structural basis for COMPASS recognition of an H2B-ubiquitinated nucleosome. Elife 9:N/A (2020). PubMed: 31922488
  • Zhang H  et al. miR-30-5p-mediated ferroptosis of trophoblasts is implicated in the pathogenesis of preeclampsia. Redox Biol 29:101402 (2020). PubMed: 31926626
  • Vigil FA  et al. Prevention of brain damage after traumatic brain injury by pharmacological enhancement of KCNQ (Kv7, "M-type") K+ currents in neurons. J Cereb Blood Flow Metab 40:1256-1273 (2020). PubMed: 31272312
  • Chen C  et al. MicroRNA-129-2-3p directly targets Wip1 to suppress the proliferation and invasion of intrahepatic cholangiocarcinoma. J Cancer 11:3216-3224 (2020). PubMed: 32231727
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-6 of 6 Abreviews or Q&A

Western blot abreview for Anti-GAPDH antibody [GA1R] - Loading Control

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (4-20% SDS-PAGE)
Loading amount
50 µg
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Jun 25 2020

Western blot abreview for Anti-GAPDH antibody [GA1R] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (E. coli Rosetta(DE3))
Gel Running Conditions
Non-reduced Denaturing (4%-12% Gel)
Loading amount
800 µg
Specification
E. coli Rosetta(DE3)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Oct 08 2019

Western blot abreview for Anti-GAPDH antibody [GA1R] - Loading Control

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (brown adipose tissue)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
50 µg
Specification
brown adipose tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Oct 17 2018

Western blot abreview for Anti-GAPDH antibody [GA1R] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (liver)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
50 µg
Specification
liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Oct 17 2018

Western blot abreview for Anti-GAPDH antibody [GA1R]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1, A549, HEK293, CEMx-174)
Gel Running Conditions
Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount
5 µg
Treatment
Cells were mixed in Ripa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification
THP-1, A549, HEK293, CEMx-174
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Read More

Dr. Francois Daubeuf

Verified customer

Submitted Oct 20 2015

Western blot abreview for Anti-GAPDH antibody [GA1R] - Loading Control

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
3.75e+006 cells
Gel Running Conditions
Reduced Denaturing (NUPAGE precasted Invitrogen 10% SDS-PAGE)
Sample
Schizosaccharomyces pombe Cell lysate - whole cell (whole cell extract)
Specification
whole cell extract
Blocking step
0.5% BSA + 5% non fat milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Jul 17 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2021 Abcam plc. All rights reserved.