Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)
![Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)](https://www.abcam.com/ps/products/125/ab125247/Images/ab125247-139416-anti-gapdh-antibody-ga1r-loading-control-western-blot.jpg "Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)")
Key features and details
- Mouse monoclonal [GA1R] to GAPDH - Loading Control
- Suitable for: WB
- Reacts with: Mouse, Rat, Rabbit, Chicken, Hamster, Human, Saccharomyces cerevisiae, Escherichia coli, Spodoptera frugiperda (SF9 cells)
- Isotype: IgG1
Overview
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Product name
Anti-GAPDH antibody [GA1R] - Loading Control
See all GAPDH primary antibodies -
Description
Mouse monoclonal [GA1R] to GAPDH - Loading Control -
Host species
Mouse -
Specificity
ab125247 recognizes native and denatured forms of GAPDH. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Chicken, Hamster, Human, Saccharomyces cerevisiae, Escherichia coli, Spodoptera frugiperda (SF9 cells)
Predicted to work with: a wide range of other species
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Immunogen
Recombinant full length protein corresponding to Rabbit GAPDH.
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Positive control
- Human, Mouse, Rat, Rabbit, Chicken and Hamster tissue lysates; BL-21 bacteria, Sf9 insect and Saccharomyces cerevisiae cell lysates
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General notes
Store at 4°C (add 0.05% NaN3) for several days to weeks.
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Protein A affinity chromatography from TCS -
Clonality
Monoclonal -
Clone number
GA1R -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (7) |
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa). with ECL.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa). with ECL. |
Target
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Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
Post-translational
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. - Information by UniProt
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Database links
- Entrez Gene: 374193 Chicken
- Entrez Gene: 2597 Human
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Entrez Gene: 100009074 Rabbit
- Entrez Gene: 24383 Rat
- Entrez Gene: 685186 Rat
- Omim: 138400 Human
see all -
Alternative names
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
Images
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Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)All lanes : Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) at 1/2000 dilution
Lane 1 : Human tissue lysate
Lane 2 : Mouse tissue lysate
Lane 3 : Rat tissue lysate
Lane 4 : Rabbit tissue lysate
Lane 5 : Chicken tissue lysate
Lane 6 : Hamster tissue lysate
Lysates/proteins at 5 µg per lane.
Developed using the ECL technique.
Predicted band size: 36 kDa -
![Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)](https://www.abcam.com/ps/products/125/ab125247/Images/ab125247-139415-anti-gapdh-antibody-ga1r-loading-control-western-blot.jpg)
Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)All lanes : Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) at 1/2000 dilution
Lane 1 : BL21 bacterial lysate
Lane 2 : Sf9 insect lysate
Lane 3 : Saccharomyces cerevisiae lysate
Lysates/proteins at 5 µg per lane.
Developed using the ECL technique.
Predicted band size: 36 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (102)
ab125247 has been referenced in 102 publications.
- Ishibashi Y et al. Correlation Between Immunoinflammatory Measures and Periostin Expression in Esophageal Squamous Cell Carcinoma: A Single-Center, Retrospective Cohort Study. Ann Surg Oncol 28:1228-1237 (2021). PubMed: 32613365
- Kashkoulinejad-Kouhi T et al. Enhancement of cisplatin sensitivity in human breast cancer MCF-7 cell line through BiP and 14-3-3? co-knockdown. Oncol Rep 45:665-679 (2021). PubMed: 33416155
- Zhang X et al. Gene correction of the CLN3 c.175G>A variant in patient-derived induced pluripotent stem cells prevents pathological changes in retinal organoids. Mol Genet Genomic Med 9:e1601 (2021). PubMed: 33497524
- Wu P et al. Circular RNA circEPSTI1 accelerates cervical cancer progression via miR-375/409-3P/515-5p-SLC7A11 axis. Aging (Albany NY) 13:4663-4673 (2021). PubMed: 33534779
- Lee HC et al. Transient prenatal ruxolitinib treatment suppresses astrogenesis during development and improves learning and memory in adult mice. Sci Rep 11:3847 (2021). PubMed: 33589712