Overview

  • Product name
    Anti-GAPDH antibody - Loading Control
    See all GAPDH primary antibodies
  • Description
    Goat polyclonal to GAPDH - Loading Control
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human GAPDH aa 175-225. The exact sequence is proprietary.
    Database link: P04406

  • Positive control
    • 293T, HeLa, Jurkat and mouse NIH 3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab157156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/2500. Predicted molecular weight: 36 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling GAPDH with ab157156 at 1/1000 (1µg/ml). Detection: DAB.
    • All lanes : Anti-GAPDH antibody - Loading Control (ab157156) at 1 µg/ml

      Lane 1 : 293T whole cell lysate at 50 µg
      Lane 2 : 293T whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 50 µg
      Lane 4 : Jurkat whole cell lysate at 50 µg
      Lane 5 : NIH 3T3 whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 36 kDa


      Exposure time: 30 seconds

    References

    This product has been referenced in:
    • Urraca N  et al. Significant transcriptional changes in 15q duplication but not Angelman syndrome deletion stem cell-derived neurons. Mol Autism 9:6 (2018). WB . Read more (PubMed: 29423132) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Escherichia coli Cell lysate - whole cell (E. coli)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    10 µg
    Specification
    E. coli
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Haibo Wang

    Verified customer

    Submitted Feb 27 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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