Anti-GAPDH antibody - Loading Control (ab157156)
Key features and details
- Goat polyclonal to GAPDH - Loading Control
- Suitable for: IHC-Fr, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GAPDH antibody - Loading Control
See all GAPDH primary antibodies -
Description
Goat polyclonal to GAPDH - Loading Control -
Host species
Goat -
Tested applications
Suitable for: IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish, Orangutan, Xenopus tropicalis
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Immunogen
Synthetic peptide within Human GAPDH aa 175-225. The exact sequence is proprietary.
Database link: P04406 -
Positive control
- 293T, HeLa, Jurkat and mouse NIH 3T3 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab157156 was affinity purified using an epitope specific to GAPDH immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157156 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/500 - 1/2000.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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| WB | (2) |
1/500 - 1/2500. Predicted molecular weight: 36 kDa.
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| Notes |
|---|
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IHC-Fr
1/500 - 1/2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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WB
1/500 - 1/2500. Predicted molecular weight: 36 kDa. |
Target
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Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
Post-translational
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. - Information by UniProt
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Database links
- Entrez Gene: 374193 Chicken
- Entrez Gene: 281181 Cow
- Entrez Gene: 2597 Human
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Entrez Gene: 100172694 Orangutan
- Entrez Gene: 396823 Pig
- Entrez Gene: 100009074 Rabbit
see all -
Alternative names
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-GAPDH antibody - Loading Control (ab157156)
Immunohistochemical analysis of frozen sections of human colon carcinoma tissue labelling GAPDH with ab157156 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-GAPDH antibody - Loading Control (ab157156)All lanes : Anti-GAPDH antibody - Loading Control (ab157156) at 1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 36 kDa
Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab157156 has been referenced in 5 publications.
- Eddy AC et al. Differential regulation of sFlt-1 splicing by U2AF65 and JMJD6 in placental-derived and endothelial cells. Biosci Rep 40:N/A (2020). PubMed: 32039444
- Li Y et al. The a-tubulin of Laodelphax striatellus mediates the passage of rice stripe virus (RSV) and enhances horizontal transmission. PLoS Pathog 16:e1008710 (2020). PubMed: 32817722
- Hope KA et al. Transcriptomic and proteomic profiling of glial versus neuronal Dube3a overexpression reveals common molecular changes in gliopathic epilepsies. Neurobiol Dis 141:104879 (2020). PubMed: 32344153
- Urraca N et al. Significant transcriptional changes in 15q duplication but not Angelman syndrome deletion stem cell-derived neurons. Mol Autism 9:6 (2018). WB . PubMed: 29423132
- Jin S et al. MiR-873 inhibition enhances gefitinib resistance in non-small cell lung cancer cells by targeting glioma-associated oncogene homolog 1. Thorac Cancer 9:1262-1270 (2018). PubMed: 30126075