Key features and details
- Rabbit polyclonal to GAPDH - Loading Control
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Cow, Human, Pig
- Isotype: IgG
Product nameAnti-GAPDH antibody - Loading Control
See all GAPDH primary antibodies
DescriptionRabbit polyclonal to GAPDH - Loading Control
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human, Pig
Predicted to work with: Mammals
Full length native protein (purified) corresponding to Pig GAPDH.
VKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLHYMVYMFQYDSTH GKFHGTVKA ENGKLVINGKAITIFQERDPANIKWGDAGATYVVESTGV FTTMEKAGAHLKGGAKRVIIS APSADAPMFVMGVNHEKYDNSLKIVSN ASCTTNCLAPLAKVIHDHFGIVEGLMTTVHAIT ATQKTVDGPSGKLWR DGRGAAQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSV VDL TCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEDQVVSCDFNSDTHSSTF DAGAGIA LNDHFVKLISWYDNEFGYSNRVVDLMVHMASKE
Database link: P00355
- HeLa cells. HeLa whole cell lysate (ab150035).
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab190304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/30000. Predicted molecular weight: 36 kDa.|
FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Sequence similaritiesBelongs to the glyceraldehyde-3-phosphate dehydrogenase family.
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
- Information by UniProt
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
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ab190304 has been referenced in 2 publications.
- Xu D et al. SIRT2 plays a novel role on progesterone, estradiol and testosterone synthesis via PPARs/LXRa pathways in bovine ovarian granular cells. J Steroid Biochem Mol Biol 185:27-38 (2019). PubMed: 30009951
- Chao T et al. Oxidative pathophysiology following volumetric muscle loss injury in a porcine model. J Appl Physiol (1985) 126:1541-1549 (2019). PubMed: 30920884