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  1. Link

    gapdh-antibody-loading-control-ab22555.pdf

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Anti-GAPDH antibody - Loading Control (ab22555)

  • Datasheet
Reviews (4)Q&A (1)References (93)

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Western blot - Anti-GAPDH antibody - Loading Control (ab22555)
  • Western blot - Anti-GAPDH antibody - Loading Control (ab22555)

Key features and details

  • Rabbit polyclonal to GAPDH - Loading Control
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Dog, Human, African green monkey
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-GAPDH antibody - Loading Control
    See all GAPDH primary antibodies
  • Description

    Rabbit polyclonal to GAPDH - Loading Control
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Dog, Human, African green monkey
    Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Turkey, Pig, Duck
  • Immunogen

    Synthetic peptide:

    DAPMFVMGVNHFKYA

    , corresponding to amino acids 126-140 of Human GAPDH.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Huntington's disease
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Isotype/Loading Controls
    • Loading Controls
    • GAPDH
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • MCF7 whole cell lysate (ab29537)
    • Mouse brain tissue lysate - total protein (ab30151)
  • Recombinant Protein

    • Recombinant Mouse GAPDH protein (ab202148)
    • Recombinant Human GAPDH protein (ab77109)
    • Recombinant human GAPDH protein (Active) (ab82633)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab22555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (4)
1/1000 - 1/5000. Detects a band of approximately 36 kDa.
Notes
WB
1/1000 - 1/5000. Detects a band of approximately 36 kDa.

Target

  • Function

    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • Sequence similarities

    Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • Post-translational
    modifications

    S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Target information above from: UniProt accession P04406 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 493876 Cat
    • Entrez Gene: 374193 Chicken
    • Entrez Gene: 281181 Cow
    • Entrez Gene: 403755 Dog
    • Entrez Gene: 2597 Human
    • Entrez Gene: 100042025 Mouse
    • Entrez Gene: 14433 Mouse
    • Entrez Gene: 396823 Pig
    • Entrez Gene: 100009074 Rabbit
    • Entrez Gene: 24383 Rat
    • Entrez Gene: 685186 Rat
    • Entrez Gene: 443005 Sheep
    • Omim: 138400 Human
    • SwissProt: Q9N2D5 Cat
    • SwissProt: P00356 Chicken
    • SwissProt: P10096 Cow
    • SwissProt: Q28259 Dog
    • SwissProt: P04406 Human
    • SwissProt: P16858 Mouse
    • SwissProt: P00355 Pig
    • SwissProt: P46406 Rabbit
    • SwissProt: P04797 Rat
    • SwissProt: Q28554 Sheep
    • Unigene: 544577 Human
    • Unigene: 592355 Human
    • Unigene: 598320 Human
    • Unigene: 304088 Mouse
    • Unigene: 309092 Mouse
    • Unigene: 317779 Mouse
    • Unigene: 343110 Mouse
    • Unigene: 392463 Mouse
    • Unigene: 392480 Mouse
    • Unigene: 414470 Mouse
    • Unigene: 458138 Mouse
    • Unigene: 458416 Mouse
    • Unigene: 475698 Mouse
    • Unigene: 129558 Rat
    • Unigene: 91450 Rat
    see all
  • Alternative names

    • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
    • aging associated gene 9 protein antibody
    • Aging-associated gene 9 protein antibody
    • BARS-38 antibody
    • cb609 antibody
    • EC 1.2.1.12 antibody
    • Epididymis secretory sperm binding protein Li 162eP antibody
    • G3P_HUMAN antibody
    • G3PD antibody
    • G3PDH antibody
    • GAPD antibody
    • GAPDH antibody
    • Glyceraldehyde 3 phosphate dehydrogenase antibody
    • glyceraldehyde 3-PDH antibody
    • Glyceraldehyde-3-phosphate dehydrogenase antibody
    • HEL-S-162eP antibody
    • KNC-NDS6 antibody
    • MGC102544 antibody
    • MGC102546 antibody
    • MGC103190 antibody
    • MGC103191 antibody
    • MGC105239 antibody
    • MGC127711 antibody
    • MGC88685 antibody
    • OCAS, p38 component antibody
    • OCT1 coactivator in S phase, 38-KD component antibody
    • peptidyl cysteine S nitrosylase GAPDH antibody
    • Peptidyl-cysteine S-nitrosylase GAPDH antibody
    • wu:fb33a10 antibody
    see all

Images

  • Western blot - Anti-GAPDH antibody - Loading Control (ab22555)
    Western blot - Anti-GAPDH antibody - Loading Control (ab22555)
    Lane 1 : Anti-GAPDH antibody - Loading Control (ab22555)
    Lanes 2-4 : Anti-GAPDH antibody - Loading Control (ab22555) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HUVEC cell lysate
    Lane 4 : PC12 cell lysate

    Lysates/proteins at 25 µg per lane.
  • Western blot - Anti-GAPDH antibody - Loading Control (ab22555)
    Western blot - Anti-GAPDH antibody - Loading Control (ab22555)
    All lanes : Anti-GAPDH antibody - Loading Control (ab22555) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : COS-7 cell lysate
    Lane 3 : MDCK cell lysate
    Lane 4 : PC-3 cell lysate
    Lane 5 : Mouse brain tissue lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ HRP conjugate at 1/4000 dilution

    Observed band size: 37 kDa why is the actual band size different from the predicted?

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (93)

Publishing research using ab22555? Please let us know so that we can cite the reference in this datasheet.

ab22555 has been referenced in 93 publications.

  • Zhao J  et al. Low-viscosity sodium alginate combined with TiO2 nanoparticles for improving neuroblastoma treatment. Int J Biol Macromol 167:921-933 (2021). PubMed: 33181214
  • Benetti A  et al. Urinary DPP4 correlates with renal dysfunction, and DPP4 inhibition protects against the reduction in megalin and podocin expression in experimental CKD. Am J Physiol Renal Physiol 320:F285-F296 (2021). PubMed: 33346727
  • Fan W  et al. Circ_0031242 Silencing Mitigates the Progression and Drug Resistance in DDP-Resistant Hepatoma Cells by the miR-924/POU3F2 Axis. Cancer Manag Res 13:743-755 (2021). PubMed: 33531841
  • Liu W  et al. LncRNA LINC01116 sponges miR-93-5p to promote cell invasion and migration in small cell lung cancer. BMC Pulm Med 21:50 (2021). PubMed: 33535997
  • Si H  et al. Anti-Tumor Effect of Celastrol on Hepatocellular Carcinoma by the circ_SLIT3/miR-223-3p/CXCR4 Axis. Cancer Manag Res 13:1099-1111 (2021). PubMed: 33574707
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-GAPDH antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (4-17%)
Loading amount
10 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Read More

Abcam user community

Verified customer

Submitted May 16 2018

Western blot abreview for Anti-GAPDH antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (brain)
Loading amount
50 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10%SDS gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Apr 30 2010

Western blot abreview for Anti-GAPDH antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
50 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Apr 15 2010

Western blot abreview for Anti-GAPDH antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Baboon Tissue lysate - whole (brain)
Loading amount
50 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Apr 08 2010

Question

Customer kindly called to find replacement product for ab36840 in use on planaria.

Read More

Abcam community

Verified customer

Asked on Jul 30 2012

Answer

Thank you very much for your interest in anti-GAPDH antibodies.

I have reviewed the products in our catalog in effort to try an find for you a product which was most similar to ab36840. I believe that the following two products offer the best alternative.

AB75834 (https://www.abcam.com/gapdh-antibody-epr1977y-ab75834.html). This rabbit monoclonal was raised against a very similar region to ab36840. Therefore it may be likely that the same epitope site will be recogized by this product.

AB22555 (https://www.abcam.com/gapdh-antibody-ab22555.html). This rabbit polyclonal was raised against an area just before that which ab36840 was. While this product will not be recognizing the same epitopes it's polyclonal nature will mean that it will recognize more sites than a monoclonal antibody.

To our knowledge, these have not been tested in planaria. Therefore, I can offer a discount off a future purchase if you buy an GAPDH antibody now, test it in planaria and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test one of these in planaria. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.

2. Purchase either by phone, fax, or online (www.abcam.com).

3. Test it in planaria.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the product turns out to be unsuitable for planaria, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

Answered on Jul 30 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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