Product nameAnti-GAPDH antibody - Loading Control
See all GAPDH primary antibodies
DescriptionRabbit polyclonal to GAPDH - Loading Control
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human, Baboon
Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Turkey, Pig, Duck
- Rat heart protein extract. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: MCF7
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab22555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/1000 - 1/5000. Detects a band of approximately 36 kDa.|
FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Sequence similaritiesBelongs to the glyceraldehyde-3-phosphate dehydrogenase family.
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
- Information by UniProt
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Lane 1 : Anti-GAPDH antibody - Loading Control (ab22555)
Lanes 2-4 : Anti-GAPDH antibody - Loading Control (ab22555) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 25 µg per lane.
ab22555 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab22555 at 5µg/ml overnight at +4°C. The secondary antibody (green) was anti-Rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Westerlund I et al. EPAS1/HIF2a correlates with features of low-risk neuroblastoma and with adrenal chromaffin cell differentiation during sympathoadrenal development. Biochem Biophys Res Commun 508:1233-1239 (2019). Read more (PubMed: 30563765) »
- Su X et al. Mechanical, nanomorphological and biological reconstruction of early-stage apoptosis in HeLa cells induced by cytochalasin B. Oncol Rep 41:928-938 (2019). Read more (PubMed: 30535459) »