Overview

  • Product name
    Anti-GAPDH antibody - Loading Control
    See all GAPDH primary antibodies
  • Description
    Rabbit polyclonal to GAPDH - Loading Control
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Baboon
    Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Turkey, Pig, Duck
  • Immunogen

    Synthetic peptide:

    DAPMFVMGVNHFKYA

    , corresponding to amino acids 126-140 of Human GAPDH.

  • Positive control
    • Rat heart protein extract. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: MCF7

Applications

Our Abpromise guarantee covers the use of ab22555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000 - 1/5000. Detects a band of approximately 36 kDa.

Target

Images

  • Lane 1 : Anti-GAPDH antibody - Loading Control (ab22555)
    Lanes 2-4 : Anti-GAPDH antibody - Loading Control (ab22555) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HUVEC cell lysate
    Lane 4 : PC12 cell lysate

    Lysates/proteins at 25 µg per lane.
  • ab22555 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab22555 at 5µg/ml overnight at +4°C. The secondary antibody (green) was anti-Rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Westerlund I  et al. EPAS1/HIF2a correlates with features of low-risk neuroblastoma and with adrenal chromaffin cell differentiation during sympathoadrenal development. Biochem Biophys Res Commun 508:1233-1239 (2019). Read more (PubMed: 30563765) »
  • Su X  et al. Mechanical, nanomorphological and biological reconstruction of early-stage apoptosis in HeLa cells induced by cytochalasin B. Oncol Rep 41:928-938 (2019). Read more (PubMed: 30535459) »
See all 36 Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (4-17%)
Loading amount
10 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Abcam user community

Verified customer

Submitted May 16 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (brain)
Loading amount
50 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10%SDS gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
50 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Baboon Tissue lysate - whole (brain)
Loading amount
50 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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