• Product name
    Anti-GAPDH antibody - Loading Control
    See all GAPDH primary antibodies
  • Description
    Rabbit polyclonal to GAPDH - Loading Control
  • Host species
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Guinea pig, Turkey, Pig, Xenopus laevis, Chimpanzee, Reptile, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide within Human GAPDH aa 250-300. The exact sequence is proprietary.
    Database link: P04406

  • Positive control
    • Whole cell lysate from HeLa and mouse NIH3T3 cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal skeletal muscle. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.


Our Abpromise guarantee covers the use of ab70699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
    • Pathway
      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
    • Sequence similarities
      Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
    • Post-translational
      S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    • Cellular localization
      Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
    • Information by UniProt
    • Database links
    • Alternative names
      • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
      • aging associated gene 9 protein antibody
      • Aging-associated gene 9 protein antibody
      • BARS-38 antibody
      • cb609 antibody
      • EC antibody
      • Epididymis secretory sperm binding protein Li 162eP antibody
      • G3P_HUMAN antibody
      • G3PD antibody
      • G3PDH antibody
      • GAPD antibody
      • GAPDH antibody
      • Glyceraldehyde 3 phosphate dehydrogenase antibody
      • Glyceraldehyde-3-phosphate dehydrogenase antibody
      • HEL-S-162eP antibody
      • KNC-NDS6 antibody
      • MGC102544 antibody
      • MGC102546 antibody
      • MGC103190 antibody
      • MGC103191 antibody
      • MGC105239 antibody
      • MGC127711 antibody
      • MGC88685 antibody
      • OCAS, p38 component antibody
      • OCT1 coactivator in S phase, 38-KD component antibody
      • peptidyl cysteine S nitrosylase GAPDH antibody
      • Peptidyl-cysteine S-nitrosylase GAPDH antibody
      • wu:fb33a10 antibody
      see all


    • All lanes : Anti-GAPDH antibody - Loading Control (ab70699) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from NIH 3T3 cells at 50 µg

      Predicted band size: 36 kDa
      Observed band size: 38 kDa
      why is the actual band size different from the predicted?

      Exposure time: 10 seconds
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling GAPDH with ab70699 at 1/200 (1µg/ml). Detection: DAB.
    • ab70699 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab70699 at 5µg/ml overnight at +4°C. The secondary antibody (green) was anti-Rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Anti-GAPDH antibody - Loading Control (ab70699) at 1/2000 dilution + Recombinant human GAPDH protein (Active) (ab82633) at 0.1 µg

      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 36 kDa

      Exposure time: 150 seconds
    • IHC image of GAPDH staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70699, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


    This product has been referenced in:
    • Chen Y  et al. Lost expression of cell adhesion molecule 1 is associated with bladder cancer progression and recurrence and its overexpression inhibited tumor cell malignant behaviors. Oncol Lett 17:2047-2056 (2019). Read more (PubMed: 30719104) »
    • Wang L  et al. MicroRNA-188-3p is involved in sevoflurane anesthesia-induced neuroapoptosis by targeting MDM2. Mol Med Rep 17:4229-4236 (2018). Read more (PubMed: 29344658) »
    See all 23 Publications for this product

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