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Anti-GAPDH antibody - Loading Control (ab83956)

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Western blot - Anti-GAPDH antibody - Loading Control (ab83956)

    Key features and details

    • Chicken polyclonal to GAPDH - Loading Control
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgY

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    Overview

    • Product name

      Anti-GAPDH antibody - Loading Control
      See all GAPDH primary antibodies
    • Description

      Chicken polyclonal to GAPDH - Loading Control
    • Host species

      Chicken
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      A 16 amino acid synthetic peptide from near the carboxy-terminus of Human GAPDH (Swiss-Prot P04406).

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.2
      Preservative: 0.02% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgY
    • Research areas

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Huntington's disease
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Other
      • Isotype/Loading Controls
      • Loading Controls
      • GAPDH
      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of carbohydrates
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Carbohydrate metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism
      • Metabolism
      • Types of disease
      • Neurodegenerative disease
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Rabbit Anti-Chicken IgY H&L (FITC) (ab6749)
      • Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
      • Rabbit Anti-Chicken IgY H&L (HRP) (ab6753)
      • Goat Anti-Chicken IgY H&L (HRP) (ab6877)
      • Goat Anti-Chicken IgY H&L (Biotin) preadsorbed (ab7117)
    • Isotype control

      • Chicken IgY - Isotype Control (ab50579)
    • Recombinant Protein

      • Recombinant human GAPDH protein (Active) (ab82633)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab83956 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 36 kDa.
    Notes
    WB
    Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 36 kDa.

    Target

    • Function

      Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
    • Pathway

      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
    • Sequence similarities

      Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
    • Post-translational
      modifications

      S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
      ISGylated.
    • Cellular localization

      Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
    • Target information above from: UniProt accession P04406 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2597 Human
      • Omim: 138400 Human
      • SwissProt: P04406 Human
      • Unigene: 544577 Human
      • Unigene: 592355 Human
      • Unigene: 598320 Human
      • Alternative names

        • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
        • aging associated gene 9 protein antibody
        • Aging-associated gene 9 protein antibody
        • BARS-38 antibody
        • cb609 antibody
        • EC 1.2.1.12 antibody
        • Epididymis secretory sperm binding protein Li 162eP antibody
        • G3P_HUMAN antibody
        • G3PD antibody
        • G3PDH antibody
        • GAPD antibody
        • GAPDH antibody
        • Glyceraldehyde 3 phosphate dehydrogenase antibody
        • glyceraldehyde 3-PDH antibody
        • Glyceraldehyde-3-phosphate dehydrogenase antibody
        • HEL-S-162eP antibody
        • KNC-NDS6 antibody
        • MGC102544 antibody
        • MGC102546 antibody
        • MGC103190 antibody
        • MGC103191 antibody
        • MGC105239 antibody
        • MGC127711 antibody
        • MGC88685 antibody
        • OCAS, p38 component antibody
        • OCT1 coactivator in S phase, 38-KD component antibody
        • peptidyl cysteine S nitrosylase GAPDH antibody
        • Peptidyl-cysteine S-nitrosylase GAPDH antibody
        • wu:fb33a10 antibody
        see all

      Images

      • Western blot - Anti-GAPDH antibody - Loading Control (ab83956)
        Western blot - Anti-GAPDH antibody - Loading Control (ab83956)
        Anti-GAPDH antibody - Loading Control (ab83956) at 1 µg/ml + HeLa Cell Lysate at 15 µg

        Predicted band size: 36 kDa
        Observed band size: 42 kDa why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (5)

      Publishing research using ab83956? Please let us know so that we can cite the reference in this datasheet.

      ab83956 has been referenced in 5 publications.

      • Wright CB  et al. Chronic Dicer1 deficiency promotes atrophic and neovascular outer retinal pathologies in mice. Proc Natl Acad Sci U S A 117:2579-2587 (2020). PubMed: 31964819
      • Bachmann SO  et al. Behavioral training rescues motor deficits in Cyfip1 haploinsufficiency mouse model of autism spectrum disorders. Transl Psychiatry 9:29 (2019). PubMed: 30664619
      • Arulsamy A  et al. Evaluation of early chronic functional outcomes and their relationship to pre-frontal cortex and hippocampal pathology following moderate-severe traumatic brain injury. Behav Brain Res 348:127-138 (2018). WB . PubMed: 29653127
      • Osório C  et al. Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. Mol Cell Neurosci 59:24-36 (2014). WB ; Mouse . PubMed: 24444792
      • McCoy DD  et al. Enhanced insulin clearance in mice lacking TRPM8 channels. Am J Physiol Endocrinol Metab 305:E78-88 (2013). PubMed: 23651844

      Customer reviews and Q&As

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