Anti-GAPDH antibody - Loading Control (ab83956)
Key features and details
- Chicken polyclonal to GAPDH - Loading Control
- Suitable for: WB
- Reacts with: Human
- Isotype: IgY
Overview
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Product name
Anti-GAPDH antibody - Loading Control
See all GAPDH primary antibodies -
Description
Chicken polyclonal to GAPDH - Loading Control -
Host species
Chicken -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
A 16 amino acid synthetic peptide from near the carboxy-terminus of Human GAPDH (Swiss-Prot P04406).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab83956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 36 kDa.
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| Notes |
|---|
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 36 kDa. |
Target
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Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
Post-translational
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. - Information by UniProt
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Database links
- Entrez Gene: 2597 Human
- Omim: 138400 Human
- SwissProt: P04406 Human
- Unigene: 544577 Human
- Unigene: 592355 Human
- Unigene: 598320 Human
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Alternative names
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
Images
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Western blot - Anti-GAPDH antibody - Loading Control (ab83956)Anti-GAPDH antibody - Loading Control (ab83956) at 1 µg/ml + HeLa Cell Lysate at 15 µg
Predicted band size: 36 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab83956 has been referenced in 5 publications.
- Wright CB et al. Chronic Dicer1 deficiency promotes atrophic and neovascular outer retinal pathologies in mice. Proc Natl Acad Sci U S A 117:2579-2587 (2020). PubMed: 31964819
- Bachmann SO et al. Behavioral training rescues motor deficits in Cyfip1 haploinsufficiency mouse model of autism spectrum disorders. Transl Psychiatry 9:29 (2019). PubMed: 30664619
- Arulsamy A et al. Evaluation of early chronic functional outcomes and their relationship to pre-frontal cortex and hippocampal pathology following moderate-severe traumatic brain injury. Behav Brain Res 348:127-138 (2018). WB . PubMed: 29653127
- Osório C et al. Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. Mol Cell Neurosci 59:24-36 (2014). WB ; Mouse . PubMed: 24444792
- McCoy DD et al. Enhanced insulin clearance in mice lacking TRPM8 channels. Am J Physiol Endocrinol Metab 305:E78-88 (2013). PubMed: 23651844