Key features and details
- Chicken polyclonal to GAPDH - Loading Control
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgY
Product nameAnti-GAPDH antibody - Loading Control
See all GAPDH primary antibodies
DescriptionChicken polyclonal to GAPDH - Loading Control
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
A 16 amino acid synthetic peptide from near the amino terminus of Human GAPDH (Swiss-Prot P04406).
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab83957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 36 kDa.|
FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Sequence similaritiesBelongs to the glyceraldehyde-3-phosphate dehydrogenase family.
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
- Information by UniProt
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
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Lane 1 : Anti-GAPDH antibody - Loading Control (ab83957) at 1 µg/ml
Lane 2 : Anti-GAPDH antibody - Loading Control (ab83957) at 2 µg/ml
All lanes : HeLa cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab83957 has been referenced in 1 publication.
- Helsby MA et al. CiteAb: a searchable antibody database that ranks antibodies by the number of times they have been cited. BMC Cell Biol 15:6 (2014). PubMed: 24528853