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Anti-GAPDH antibody - Loading Control (ab9483)

  • Datasheet
  • SDS
Reviews (7) Submit a question References (117)

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Western blot - Anti-GAPDH antibody - Loading Control (ab9483)
  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9483)
  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9483)

Key features and details

  • Goat polyclonal to GAPDH - Loading Control
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Primary
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Anti-GBA antibody [EPR5143(3)] (ab128879)
Peptide
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Human beta Actin peptide (ab13772)
Protein
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Recombinant Mouse GAPDH protein (ab202148)

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Overview

  • Product name

    Anti-GAPDH antibody - Loading Control
    See all GAPDH primary antibodies
  • Description

    Goat polyclonal to GAPDH - Loading Control
  • Host species

    Goat
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Full length native protein (purified) corresponding to Human GAPDH.

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HeLa; NIH3T3. This antibody also gave a positive signal in Human brain tissue lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide

    This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Huntington's disease
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Isotype/Loading Controls
    • Loading Controls
    • GAPDH
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Mouse GAPDH protein (ab202148)
    • Recombinant Human GAPDH protein (ab77109)
    • Recombinant human GAPDH protein (Active) (ab82633)
  • Related Products

    • Anti-SDHB antibody [21A11AE7] (ab14714)
    • Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    • Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    • Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224)
    • Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)
    • Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
Application Abreviews Notes
WB (5)
1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 35.8 kDa).
ICC/IF (1)
Use a concentration of 1 µg/ml.
Notes
WB
1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 35.8 kDa).
ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Function

    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • Sequence similarities

    Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • Post-translational
    modifications

    S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Target information above from: UniProt accession P04406 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2597 Human
    • Entrez Gene: 100042025 Mouse
    • Entrez Gene: 14433 Mouse
    • Omim: 138400 Human
    • SwissProt: P04406 Human
    • SwissProt: P16858 Mouse
    • Unigene: 544577 Human
    • Unigene: 592355 Human
    • Unigene: 598320 Human
    • Unigene: 304088 Mouse
    • Unigene: 309092 Mouse
    • Unigene: 317779 Mouse
    • Unigene: 343110 Mouse
    • Unigene: 392463 Mouse
    • Unigene: 392480 Mouse
    • Unigene: 414470 Mouse
    • Unigene: 458138 Mouse
    • Unigene: 458416 Mouse
    • Unigene: 475698 Mouse
    see all
  • Alternative names

    • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
    • aging associated gene 9 protein antibody
    • Aging-associated gene 9 protein antibody
    • BARS-38 antibody
    • cb609 antibody
    • EC 1.2.1.12 antibody
    • Epididymis secretory sperm binding protein Li 162eP antibody
    • G3P_HUMAN antibody
    • G3PD antibody
    • G3PDH antibody
    • GAPD antibody
    • GAPDH antibody
    • Glyceraldehyde 3 phosphate dehydrogenase antibody
    • glyceraldehyde 3-PDH antibody
    • Glyceraldehyde-3-phosphate dehydrogenase antibody
    • HEL-S-162eP antibody
    • KNC-NDS6 antibody
    • MGC102544 antibody
    • MGC102546 antibody
    • MGC103190 antibody
    • MGC103191 antibody
    • MGC105239 antibody
    • MGC127711 antibody
    • MGC88685 antibody
    • OCAS, p38 component antibody
    • OCT1 coactivator in S phase, 38-KD component antibody
    • peptidyl cysteine S nitrosylase GAPDH antibody
    • Peptidyl-cysteine S-nitrosylase GAPDH antibody
    • wu:fb33a10 antibody
    see all

Images

  • Western blot - Anti-GAPDH antibody - Loading Control (ab9483)
    Western blot - Anti-GAPDH antibody - Loading Control (ab9483)
    All lanes : Anti-GAPDH antibody - Loading Control (ab9483) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Rabbit polyclonal to Goat IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 35.8 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9483)
    Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9483)
    ICC/IF image of ab9483 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9483, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 1µg/ml.
  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9483)
    Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9483)This image is courtesy of an Abreview submitted by Kirk McManus

    ab9483 staining GAPDH in HeLa by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehydeand. Samples were incubated with primary antibody (1/200) for 30 minutes. An undiluted Alexa Fluor® 568-conjugated Donkey anti-goat polyclonal was used as the secondary antibody.

    See Abreview

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (117)

    Publishing research using ab9483? Please let us know so that we can cite the reference in this datasheet.

    ab9483 has been referenced in 117 publications.

    • Zhang J  et al. Knockdown of RAP2A gene expression suppresses cisplatin resistance in gastric cancer cells. Oncol Lett 19:350-358 (2020). PubMed: 31897147
    • Chen FX  et al. Inflammation-dependent downregulation of miR-532-3p mediates apoptotic signaling in human sarcopenia through targeting BAK1. Int J Biol Sci 16:1481-1494 (2020). PubMed: 32226296
    • Alanazi AM  et al. Liposomal Resveratrol and/or Carvedilol Attenuate Doxorubicin-Induced Cardiotoxicity by Modulating Inflammation, Oxidative Stress and S100A1 in Rats. Antioxidants (Basel) 9:N/A (2020). PubMed: 32079097
    • Mamriev D  et al. A small-molecule ARTS mimetic promotes apoptosis through degradation of both XIAP and Bcl-2. Cell Death Dis 11:483 (2020). PubMed: 32587235
    • Kravchenko DS  et al. PDLIM4/RIL-mediated regulation of Src and malignant properties of breast cancer cells. Oncotarget 11:22-30 (2020). PubMed: 32002121
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-GAPDH antibody - Loading Control

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (RPE cells)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    30 µg
    Specification
    RPE cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 04 2019

    Western blot abreview for Anti-GAPDH antibody - Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (U2OS osteosarcoma)
    Specification
    U2OS osteosarcoma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 21 2014

    Western blot abreview for Anti-GAPDH antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (skeletal muscle)
    Loading amount
    50 µg
    Specification
    skeletal muscle
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 25 2012

    Western blot abreview for Anti-GAPDH antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (aorta)
    Loading amount
    20 µg
    Specification
    aorta
    Gel Running Conditions
    Reduced Denaturing (Bis-tris 4-12%)
    Blocking step
    Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 20 2010

    Western blot abreview for Anti-GAPDH antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Loading amount
    50 µg
    Specification
    U2OS cells
    Gel Running Conditions
    Reduced Denaturing (4-20% tris-glycine)
    Blocking step
    normal horse serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Dr. Natalie Farny

    Verified customer

    Submitted Oct 23 2009

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GAPDH antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain sections)
    Specification
    Brain sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Read More

    Dr. Sophie Pezet

    Verified customer

    Submitted Jul 31 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GAPDH antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Oct 03 2006

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