Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GAPDH antibody

Average
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No

Other product details

Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS + 0.3 % Triton X100

Secondary antibody

Name
Non-Abcam antibody was used: Chicken anti-goat Cy3
Host species: Chicken
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/300

Additional data

Additional Notes
The antibody stains the membrane of many cells in the mouse brain.

The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.

Dr. Sophie Pezet

Verified customer

Submitted Jul 31 2009

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