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Immunohistochemistry (PFA perfusion fixed frozen sections)
Mouse Tissue sections (Brain sections)
Antigen retrieval step
Other product details
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS + 0.3 % Triton X100
Non-Abcam antibody was used: Chicken anti-goat Cy3
Host species: Chicken
Conjugation: Cy3 ®
The antibody stains the membrane of many cells in the mouse brain.
The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.
Dr. Sophie Pezet
Submitted Jul 31 2009