Western blot abreview for Anti-GAPDH antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Loading amount
50 µg
Specification
U2OS cells
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycine)
Blocking step
normal horse serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/10000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 5% normal horse serum in PBS

Secondary antibody

Name
Non-Abcam antibody was used: donkey-anti-goat-HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
2 second(s)
Bands
Specific: 37 kDa
Positive control
GAPDH siRNA
Negative control
control siRNA

Additional data

Additional Notes
human derived U2OS cells were treated siRNA targeting the following: Lane 1, control Lane 2, Upf1 Lane 3, GAPDH. After 4 days, cells were lysed and processed for Western blotting with the antibodies as indicated. Upf1 antibody is Abcam 10510 (see product page for Abreview). Treatment with GAPDH siRNA decreased the GAPDH Western blot signal as expected. This antibody is really strong - at a 1:10,000 dilution and stringent washing I still only needed to expose the film for 1-2 seconds!

Dr. Natalie Farny

Verified customer

Submitted Oct 23 2009

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