human derived U2OS cells were treated siRNA targeting the following: Lane 1, control Lane 2, Upf1 Lane 3, GAPDH. After 4 days, cells were lysed and processed for Western blotting with the antibodies as indicated. Upf1 antibody is Abcam 10510 (see product page for Abreview). Treatment with GAPDH siRNA decreased the GAPDH Western blot signal as expected. This antibody is really strong - at a 1:10,000 dilution and stringent washing I still only needed to expose the film for 1-2 seconds!
Dr. Natalie Farny
Submitted Oct 23 2009
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