Western blot abreview for Anti-GAPDH antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (aorta)
Loading amount
20 µg
Specification
aorta
Gel Running Conditions
Reduced Denaturing (Bis-tris 4-12%)
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 20°C

Other product details

Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey blocking buffer

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-goat IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 700DX
Dilution
1/15000

Detection

Detection method
Licor Odyssey IR
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 37 kDa

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Submitted May 20 2010

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