Western blot abreview for Anti-GAPDH antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (skeletal muscle)
Loading amount
50 µg
Specification
skeletal muscle
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS

Secondary antibody

Name
Non-Abcam antibody was used: anti-goat IgG HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 37 kDa

Additional data

PubMed ID
22733784: View

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Submitted Sep 25 2012

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