Western blot abreview for Anti-GAPDH antibody - Loading Control

Excellent
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (U2OS osteosarcoma)
Specification
U2OS osteosarcoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/3000

Secondary antibody

Name
Non-Abcam antibody was used: Li-CoR Donkey anti goat Antibody
Host species: Donkey
Clonality: Monoclonal
Conjugation: IRDye­ 800CW
Dilution
1/15000

Detection

Detection method
Li-Cor Odyssey Scanner
Bands
Specific: 37 kDa
Exposure
10 minute(s) and 0 second(s)

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Submitted Feb 21 2014

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