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    gapdh-antibody-loading-control-ab9485.pdf

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Anti-GAPDH antibody - Loading Control (ab9485)

  • Datasheet
  • SDS
Reviews (86)Q&A (11)References (1630)

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Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
  • Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (ab9485)
  • Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9485)
  • Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
  • Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9485)
  • Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
  • ELISA - Anti-GAPDH antibody - Loading Control (ab9485)

Key features and details

  • Rabbit polyclonal to GAPDH - Loading Control
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Primary
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Anti-SLC34A3 antibody [EPR9588] (ab155986)
Protein
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Recombinant Mouse GAPDH protein (ab202148)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-GAPDH antibody - Loading Control
    See all GAPDH primary antibodies
  • Description

    Rabbit polyclonal to GAPDH - Loading Control
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Full length native protein (purified) corresponding to Human GAPDH.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Huntington's disease
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Isotype/Loading Controls
    • Loading Controls
    • GAPDH
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • HRP Anti-GAPDH antibody - Loading Control (ab9385)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Mouse GAPDH protein (ab202148)
    • Recombinant Human GAPDH protein (ab77109)
    • Recombinant human GAPDH protein (Active) (ab82633)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-SDHB antibody [21A11AE7] (ab14714)
    • Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    • Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    • Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224)
    • Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Mouse
Human
IHC-P
Human
WB
Human
All applications
Rat
Chicken
Dog
Saccharomyces cerevisiae
Xenopus laevis
Schizosaccharomyces pombe
African green monkey
Application Abreviews Notes
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (72)
1/2500. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa).

Some customers have experienced that milk significantly decreases the signal in WB compared to BSA. In-house we use BSA.

We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) secondary antibody.

ICC/IF (6)
Use a concentration of 5 µg/ml.

We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody.

Notes
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/2500. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa).

Some customers have experienced that milk significantly decreases the signal in WB compared to BSA. In-house we use BSA.

We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) secondary antibody.

ICC/IF
Use a concentration of 5 µg/ml.

We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody.

Target

  • Function

    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • Sequence similarities

    Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • Post-translational
    modifications

    S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Target information above from: UniProt accession P04406 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 374193 Chicken
    • Entrez Gene: 403755 Dog
    • Entrez Gene: 2597 Human
    • Entrez Gene: 100042025 Mouse
    • Entrez Gene: 14433 Mouse
    • Entrez Gene: 24383 Rat
    • Entrez Gene: 685186 Rat
    • Entrez Gene: 380259 Xenopus laevis
    • Omim: 138400 Human
    • SwissProt: P00356 Chicken
    • SwissProt: Q28259 Dog
    • SwissProt: P04406 Human
    • SwissProt: P16858 Mouse
    • SwissProt: P04797 Rat
    • SwissProt: P51469 Xenopus laevis
    • Unigene: 544577 Human
    • Unigene: 592355 Human
    • Unigene: 598320 Human
    • Unigene: 304088 Mouse
    • Unigene: 309092 Mouse
    • Unigene: 317779 Mouse
    • Unigene: 343110 Mouse
    • Unigene: 392463 Mouse
    • Unigene: 392480 Mouse
    • Unigene: 414470 Mouse
    • Unigene: 458138 Mouse
    • Unigene: 458416 Mouse
    • Unigene: 475698 Mouse
    • Unigene: 129558 Rat
    • Unigene: 91450 Rat
    • Unigene: 995 Xenopus laevis
    see all
  • Alternative names

    • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
    • aging associated gene 9 protein antibody
    • Aging-associated gene 9 protein antibody
    • BARS-38 antibody
    • cb609 antibody
    • EC 1.2.1.12 antibody
    • Epididymis secretory sperm binding protein Li 162eP antibody
    • G3P_HUMAN antibody
    • G3PD antibody
    • G3PDH antibody
    • GAPD antibody
    • GAPDH antibody
    • Glyceraldehyde 3 phosphate dehydrogenase antibody
    • glyceraldehyde 3-PDH antibody
    • Glyceraldehyde-3-phosphate dehydrogenase antibody
    • HEL-S-162eP antibody
    • KNC-NDS6 antibody
    • MGC102544 antibody
    • MGC102546 antibody
    • MGC103190 antibody
    • MGC103191 antibody
    • MGC105239 antibody
    • MGC127711 antibody
    • MGC88685 antibody
    • OCAS, p38 component antibody
    • OCT1 coactivator in S phase, 38-KD component antibody
    • peptidyl cysteine S nitrosylase GAPDH antibody
    • Peptidyl-cysteine S-nitrosylase GAPDH antibody
    • wu:fb33a10 antibody
    see all

Images

  • Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
    Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
    All lanes : Anti-GAPDH antibody - Loading Control (ab9485) at 1/2500 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) secondary antibody at 1/10000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa



    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab9485 overnight at 4°C. Antibody binding was detected using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) secondary antibody at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
    Western blot - Anti-GAPDH antibody - Loading Control (ab9485)Image from Wu T et al., PLoS One, 14(4), Fig 3.; doi: 10.1371/journal.pone.0216042. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

    HEK293 cells stably transfected with pINDUCER10-shNF90/NF110 (D2) or pINDUCER10-shNF45 (D5) were treated without or with doxycycline for 96 h, then serum starved for 12 h and treated with PMA (20 ng/mL) for 2 h. Protein lysates (20 µg/lane) were separated by SDS-PAGE and transferred to PVDF membranes. 

    Loading control: Rabbit polyclonal to GAPDH (ab9485) at 1/1000 dilution.

    Secondary antibodies (HRP) were used at 1/10,000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (ab9485)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (ab9485)

    IHC image of ab9485 staining GAPDH in human pancreas formalin fixed paraffin embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9485, 5μg/ml working concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
    Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
    All lanes : Anti-GAPDH antibody - Loading Control (ab9485) at 1 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HEK-293 cell lysate
    Lane 5 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 37 kDa



    Western blot image using 4-20% Optiblot gel with the Prism Ultra Protein Ladder (ab116028) 5µl used. We recommend using our ECL substrate kit (ab65623).
    20ug of Lysate per lane and detection using ab9485 diluted to 1ug/ml.
    Lane 1: HeLa cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: A431 cell lysate
    Lane 4: HEK-293 cell lysate
    Lane 5: HepG2 cell lysate.

  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9485)
    Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9485)

    ab9485 staining GAPDH in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab9485 at 5μg/ml and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 2 μg/ml (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) secondary antibody at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.

    Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

  • Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
    Western blot - Anti-GAPDH antibody - Loading Control (ab9485)This image is courtesy of an anonymous Abreview
    All lanes : Anti-GAPDH antibody - Loading Control (ab9485) at 1/1000 dilution

    Lane 1 : Mouse hepatocytes - untreated
    Lane 2 : Mouse hepatocytes - treated with LPS (100 ng/mL) for 1 hour
    Lane 3 : Mouse hepatocytes - treated with LPS (100 ng/mL) for 12 hours

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit secondary antibody (HRP) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 37 kDa


    Exposure time: 1 minute


    Primary incubation: 16 hours at 4°C

    Blocking: 5% milk for 1 hour at room temperature

     

    See Abreview

  • Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
    Western blot - Anti-GAPDH antibody - Loading Control (ab9485)This image is a courtesy of Anonymous Abreview
    All lanes : Anti-GAPDH antibody - Loading Control (ab9485) at 1/2500 dilution

    Lane 1 : Lysate prepared from human Huh-7 cells at 2 µg
    Lane 2 : Lysate prepared from human Huh-7 cells at 20 µg

    Secondary
    All lanes : HRP-conjugated sheep polyclonal to rabbit IgG at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?


    Exposure time: 5 minutes

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9485)
    Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9485)

    ab9485 staining GAPDH in NIH3T3 cells. The cells were fixed with 4% formaldehyde (10min),  permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab9485 at 5μg/ml and ab195889 at 1/250 overnight at +4°C, followed by a further incubation at room temperature for 1h with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
    Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
    Anti-GAPDH antibody - Loading Control (ab9485) at 1/1000 dilution + Mouse Embryonic lung whole tissue lysate at 30 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 37 kDa


    Exposure time: 15 seconds

    See Abreview

  • ELISA - Anti-GAPDH antibody - Loading Control (ab9485)
    ELISA - Anti-GAPDH antibody - Loading Control (ab9485)

    ab9485 detecting GAPDH in Human fibroblasts samples by indirect ELISA. Samples were blocked with 1% BSA for 1 hour at 25°C and incubated with the primary antibody (1/2500) for 1 hour at 25°C. An alkaline phosphatase Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    The capture of ab8245 was 1/2000. Detection of ab9485 was 1/2500.

    See Abreview

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1630)

Publishing research using ab9485? Please let us know so that we can cite the reference in this datasheet.

ab9485 has been referenced in 1630 publications.

  • Jiang L  et al. Inhibition of microRNA-103 attenuates inflammation and endoplasmic reticulum stress in atherosclerosis through disrupting the PTEN-mediated MAPK signaling. J Cell Physiol 235:380-393 (2020). PubMed: 31232476
  • Bai J  et al. lncRNA SNHG1 cooperated with miR-497/miR-195-5p to modify epithelial-mesenchymal transition underlying colorectal cancer exacerbation. J Cell Physiol 235:1453-1468 (2020). PubMed: 31276207
  • Liu J  et al. The inhibition of miR-101a-3p alleviates H/R injury in H9C2 cells by regulating the JAK2/STAT3 pathway. Mol Med Rep 21:89-96 (2020). PubMed: 31746349
  • Zhao B  et al. Rhizoma Paridis total saponins alleviate H2O2-induced oxidative stress injury by upregulating the Nrf2 pathway. Mol Med Rep 21:220-228 (2020). PubMed: 31746361
  • Nakano Y  et al. Clinical importance of long non-coding RNA LINC00460 expression in EGFR-mutant lung adenocarcinoma. Int J Oncol 56:243-257 (2020). PubMed: 31789388
View all Publications for this product

Customer reviews and Q&As

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1-10 of 97 Abreviews or Q&A

Western blot abreview for Anti-GAPDH antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Loading amount
10 µg
Specification
HEK293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

Submitted Dec 21 2020

Western blot abreview for Anti-GAPDH antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
Glioblastoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Dec 07 2020

Western blot abreview for Anti-GAPDH antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
HEK293T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Nov 11 2020

Western blot abreview for Anti-GAPDH antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (primary cardiomyocytes)
Gel Running Conditions
Reduced Denaturing (4-12 %)
Loading amount
20 µg
Treatment
LPS 1 µg/ml
Specification
primary cardiomyocytes
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Dr. Barbara Thaler

Verified customer

Submitted Jul 04 2019

Western blot abreview for Anti-GAPDH antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (HL-1 cardiomyocytes)
Gel Running Conditions
Reduced Denaturing (4-12 %)
Loading amount
40 µg
Treatment
LPS 1 µg/ml
Specification
HL-1 cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Dr. Barbara Thaler

Verified customer

Submitted Jul 03 2019

Western blot abreview for Anti-GAPDH antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary murine microglia)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Treatment
1µM LPA for different time points
Specification
Primary murine microglia
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Dr. Lisha Joshi

Verified customer

Submitted Jun 12 2019

Western blot abreview for Anti-GAPDH antibody - Loading Control

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (PNT2 prostate tissue)
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
20 µg
Specification
PNT2 prostate tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Jan 08 2019

Western blot abreview for Anti-GAPDH antibody - Loading Control

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse brain primary culture)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
400000 cells
Specification
Mouse brain primary culture
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Read More

Abcam user community

Verified customer

Submitted Dec 31 2018

Western blot abreview for Anti-GAPDH antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human A431 cells)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Human A431 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Nov 22 2018

Western blot abreview for Anti-GAPDH antibody - Loading Control

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%)
Loading amount
35 µg
Treatment
5% ethanol for 10 days
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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