Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP (prostate cancer cell line))
Loading amount
10 µg
Specification
LNCaP (prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing (7% tris -acetate gel)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Dilution
1/20000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% BSA in TBS-tween

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL+
Exposure
2 second(s)
Bands
Specific: 40 kDa

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Submitted Jul 14 2008

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