Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
1 hour(s) and 0 minute(s) · Diluent: TTBS
Specific: ~40, 35 kDa Non-specific: several kDa
Non-specific bands could probably be dealt with by using antibody at a greater dilution (we have used successfully -and more cleanly- at 1/20,000, but not on these samples). Lanes 1-6 = German shepherd, lane 7 = beagle, all heart (ventricle) lysates extracted in RIPA buffer.
Dr. Teresa Gunn
Submitted Nov 18 2008
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