Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

Excellent
Abreviews
Application
Western blot
Sample
Dog Tissue lysate - whole (heart)
Loading amount
30 µg
Specification
heart
Gel Running Conditions
Reduced Denaturing (4% stacking, 12% resolving)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/6000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: TTBS

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL+
Exposure
3 second(s)
Bands
Specific: ~40, 35 kDa Non-specific: several kDa

Additional data

Additional Notes
Non-specific bands could probably be dealt with by using antibody at a greater dilution (we have used successfully -and more cleanly- at 1/20,000, but not on these samples).
Lanes 1-6 = German shepherd, lane 7 = beagle, all heart (ventricle) lysates extracted in RIPA buffer.

Dr. Teresa Gunn

Verified customer

Submitted Nov 18 2008

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