Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (heart)
Loading amount
30 µg
Specification
heart
Gel Running Conditions
Reduced Denaturing (4% stacking, 12% resolving)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Dilution
1/6000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: TTBS

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: ~35, 40 kDa

Dr. Teresa Gunn

Verified customer

Submitted Nov 17 2008

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