Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

Inconclusive
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
3 µg
Specification
HEK293
Treatment
X-rays (5Gy)
Gel Running Conditions
Reduced Denaturing (17.5%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s)

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL
Exposure
15 minute(s) and 0 second(s)
Positive control
i rebloted the membrane with an anti-H2B and with coomassie as well: the WB did perfectly work, but not the anti-GAPDH antibody-HRP coupled (ab9484)

Additional data

Additional Notes
Does not work at all...I highly recommend ab9485 instead...
Abcam response
Thank you for your honest and valuable feedback. I am sorry you are experiencing problems with this antibody. It is most likely due to a loss of HRP activity. If you purchased this antibody within the last four months, I would be happy to offer you a replacement vial free of charge.

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Verified customer

Submitted Jan 29 2009

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