Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (HeLa cells)
Loading amount
500000 cells
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/10000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: in PBS with 5% BSA

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 35, 32 kDa

Additional data

Additional Notes
used as a loading control

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Submitted Nov 12 2010

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