Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
30000 cells
Specification
293T
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycine)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Dilution
1/5000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: 36 kDa
Positive control
Untreated 293T cells

Additional data

Additional Notes
Even though multiple reviewers have recommended against using milk when using this antibody, 5% milk was used in this case with excellent results. A clean and clear single specific band was observed with just 5 seconds of exposure on film.

Abcam user community

Verified customer

Submitted Jun 10 2011

Sign up