Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Loading amount
50 µg
Specification
Heart
Gel Running Conditions
Non-reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Other product details

Dilution
1/8000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 17°C · Diluent: 1% BSA

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL
Exposure
1 second(s)
Bands
Specific: 36 kDa Non-specific: No kDa
Positive control
cytosolic cell lysate
Negative control
Nuclear cell lysate

Additional data

Additional Notes
Fantastic antibody

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Submitted Mar 30 2012

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