Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Total Heart)
Loading amount
20 µg
Specification
Total Heart
Gel Running Conditions
Reduced Non-Denaturing (Native) (12% tris glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C

Other product details

Dilution
1/5000
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 18°C · Diluent: 1% milk

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL
Exposure
2 second(s)
Bands
Specific: 36 kDa Non-specific: 0 kDa

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Submitted Mar 29 2012

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