Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

Excellent
Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (Skeletal muscle)
Loading amount
20 µg
Specification
Skeletal muscle
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Dilution
1/5000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 3% milk in TBS-Tween (0.005%)

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL+
Exposure
3 second(s)
Bands
Specific: 36 kDa

Additional data

Additional Notes
Nitrocellulose membrane
1% SDS lysis buffer with 1:100 protease inhibitors

Dr. G.K Sakellariou

Verified customer

Submitted May 14 2013

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