Western blot abreview for Anti-GAPDH [mAbcam 9484] antibody - Loading Control (HRP)

Excellent
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% gel)
Sample
Rat Tissue lysate - other (Cytosolic fractions from skeletal muscles)
Specification
Cytosolic fractions from skeletal muscles
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 3% milk
Dilution
1/5000

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL+
Bands
Specific: 36 kDa
Exposure
20 second(s)

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Submitted May 31 2014

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