Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP)

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Abreviews
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (12% polyacrylamide)
Sample
Human Cell lysate - whole cell (Huh-7.5 liver cells)
Specification
Huh-7.5 liver cells
Treatment
siRNA treated cells + untreated control lysate
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + Tween 0,2% + BSA 5%
Dilution
1/10000

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL
Bands
Specific: 37 kDa
Exposure
20 second(s)
Positive control
siRNA raised against GAPDH : expression of GAPDH is well diminished since the band observed lost intensity.

Additional data

Additional Notes
Lanes : 1. Huh-7.5 cell lysate 2. Huh-7.5 treated with siRNA raised against GAPDH 3. Huh-7.5 treated with non-targeting siRNA 4. Huh-7.5 treated with siRNA targeting PAK1

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Submitted Sep 05 2014

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