Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP)

Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Incubation time
30 minute(s) · Temperature: 25°C · Diluent: 3% milk (TBS-tween)
Dilution
1/30000

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL+
Exposure
2 second(s)
Bands
Specific: 36 kDa

Additional data

Additional Notes
Lysis Buffer: Ripa buffer + protease inhibitors (1:1000)

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Submitted Mar 17 2016

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