Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (TF-1 (human, lymphoid, erythroleukemia) cells)
Loading amount
30 µg
Specification
TF-1 (human, lymphoid, erythroleukemia) cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%

Other product details

Dilution
1/10000
Incubation time
30 minute(s)

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 40 kDa

Additional data

Additional Notes
Figure
Lane 1 TF-1 cell lysate
Lanes 2-4 TNF treated TF-1 cell lysates

Samples lysed in RIPA buffer (with Protease inhibitor cocktail added) for 45 mins on ice, Supernatant removed and mixed with 2x SDS page loading buffer. Samples boiled and loaded.

Dr. Alison Taylor

Verified customer

Submitted Aug 30 2006

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