Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

Inconclusive
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK)
Loading amount
20 µg
Specification
HEK
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%

Other product details

Dilution
1/5000
Incubation time
12 hour(s) and 0 minute(s)

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ecl
Exposure
20 minute(s) and 0 second(s)
Positive control
I had other primary antibodies on the same membrane that worked
Negative control
-

Additional data

Abcam response
Thank you for your honest feedback regarding ab9842.

In our laboratory the membrane is blocked in 5%BSA for 1 hour. Following concerning customer feedback on the lack of signal with some of the vials (and other customers being very satisfied with the same lot) we have tested the membrane with a milk blocking step and found that it will significantly decrease the signal and is therefore not a suitable blocking agent for this antibody (images of our tests are available on the datasheet of the non-HRP conjugated version of the antibody, ab9484).

Dr. Ioana Carcea

Verified customer

Submitted Oct 09 2006

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