Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

Good
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (mesenchymal stem cell)
Loading amount
10 µg
Specification
mesenchymal stem cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Dilution
1/10000
Incubation time
14 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: sigma
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ecl
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 40 kDa
Positive control
Equal loading control for two samples
Negative control
N/A

Additional data

Additional Notes
Very strong signal
ab9482

Order Ref. MMLL126834
Abcam response
Thank you for your feedback. We are glad you are seeing good results with this antibody. We would like to note that some customers have been having problems visualizing the bands with this antibody when blocking in milk, so we no longer recommend milk as a blocking buffer for this product.

Abcam user community

Verified customer

Submitted Oct 31 2006

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