Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK-293 Cell Line)
Loading amount
50000 cells
Specification
HEK-293 Cell Line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%

Other product details

Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s)

Secondary antibody

Secondary antibody
None used

Detection

Detection method
CDP Star in Western Breeze Kit
Exposure
10 second(s)
Bands
Specific: 35 kDa

Additional data

Additional Notes
The image susggests a slightly lower than expected band but the band is at the limit of a 3-8% Tris Acetate gel on which I was focussing on a much bigger protein. The markers in the gel are not recommended for such low molecular weights but the band is the only one visible and I am confident that it is GAPDH.

Dr. Alan Hendrick

Verified customer

Submitted Dec 18 2006

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