Key features and details
- Detection method: Colorimetric
- Platform: Microplate (12 x 8 well strips)
- Sample type: Inhibitor compounds
Product nameGAPDH Inhibitor Screening Kit (Colorimetric)
See all GAPDH kits
Sample typeInhibitor compounds
Assay durationMultiple steps standard assay
In this GAPDH Inhibitor Screening Kit, Glyceraldehyde 3-Phosphate is oxidized by GAPDH to generate 1,3-Bisphosphoglycerate and NADH, which reduces the probe thereby generating a strong absorbance at 450 nm. In the presence of GAPDH inhibitors, the reactions are impeded, thus decreasing the rate and/or extent of generation of GAPDH-dependent absorbance at OD 450 nm. This kit provides a sensitive, quick, and easy method for screening potential inhibitors of GAPDH. GAPDH Inhibitor Control is included in the kit to compare the efficacy of test inhibitors. The assay is high-throughput adaptable and can be performed in less than 30 min.
This product is manufactured by BioVision, an Abcam company and was previously called K2043 Human GAPDH Inhibitor Screening Kit (Colorimetric). K2043-100 is the same size as the 100 test size of ab283369.
Glucose 3-Phosphate Dehydrogenase (GAPDH; EC 18.104.22.168) is a cytosolic enzyme, which catalyzes the conversion of Glyceraldehyde 3- Phosphate to 1,3-Bisphosphoglycerate (BPG) with the reduction of NAD to NADH in the glycolytic pathway. GAPDH is a multifunctional protein involved in many intracellular processes such as apoptosis, membrane trafficking, iron metabolism, nuclear translocation etc. Although GAPDH is considered as housekeeping gene, increased GAPDH activity has been detected in various human cancers. Additionally, knockdown of GAPDH has been shown to decrease cell proliferation, cell migration and metastasis in in vitro experiments. Therefore, targeting this key enzyme is essential for developing novel therapeutics for treating cancer.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests GAPDH Assay Buffer WM 1 x 25ml GAPDH Developer Red 1 vial GAPDH Inhibitor Control Purple 1 x 20µl GAPDH Reconstitution Buffer White 1 x 1.5ml GAPDH Substrate Blue 1 x 200µl Human GAPDH Brown 1 vial
ab283369 has not yet been referenced specifically in any publications.