Anti-GAPDS antibody (ab153802)
Key features and details
- Rabbit polyclonal to GAPDS
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GAPDS antibody
See all GAPDS primary antibodies -
Description
Rabbit polyclonal to GAPDS -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human GAPDS aa 161-391.
Database link: O14556 -
Positive control
- ICC/IF: MCF7 cells WB: NIH/3T3, MCF7 cells, mouse testis extract. IHC-P: Human gastric cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/10000. Predicted molecular weight: 45 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/10000. Predicted molecular weight: 45 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
Target
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Function
May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility. -
Tissue specificity
Testis specific. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
Domain
The testis-specific N-terminal extension mediates tight association with the cytoskeletal fibrous sheath of the spermatozoa flagellum, possibly via interchain disulfide-bonding of Cys-21 with sheath components. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 532231 Cow
- Entrez Gene: 26330 Human
- Entrez Gene: 14447 Mouse
- Entrez Gene: 66020 Rat
- Omim: 609169 Human
- SwissProt: Q2KJE5 Cow
- SwissProt: O14556 Human
- SwissProt: Q64467 Mouse
see all -
Alternative names
- G3PT_HUMAN antibody
- GAPD2 antibody
- GAPDH 2 antibody
see all
Images
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Anti-GAPDS antibody (ab153802) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 45 kDa
10% SDS PAGE -
Paraffin embedded human gastric cancer tissue stained for GAPDHS using ab153802 at 1/500 dilution in immunohistochemical analysis.
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Anti-GAPDS antibody (ab153802) at 1/5000 dilution + NIH/3T3 whole cell lysate at 30 µg
Predicted band size: 45 kDa
10% SDS PAGE -
Immunofluorescent analysis of methanol-fixed MCF7 cells labeling GAPDS with ab153802 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
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Anti-GAPDS antibody (ab153802) at 1/500 dilution + Mouse testis tissue extract at 50 µg
Predicted band size: 45 kDa10% SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab153802 has been referenced in 6 publications.
- He J et al. Circular RNA MAPK4 (circ-MAPK4) inhibits cell apoptosis via MAPK signaling pathway by sponging miR-125a-3p in gliomas. Mol Cancer 19:17 (2020). PubMed: 31992303
- Cheng L et al. EFEMP1 Overexpression Contributes to Neovascularization in Age-Related Macular Degeneration. Front Pharmacol 11:547436 (2020). PubMed: 33584252
- Liu X et al. Aberrant expression of sperm-specific glycolytic enzymes are associated with poor sperm quality. Mol Med Rep 19:2471-2478 (2019). PubMed: 30720094
- Yan L et al. IL-21R functions as an oncogenic factor and is regulated by the lncRNA MALAT1/miR-125a-3p axis in gastric cancer. Int J Oncol 54:7-16 (2019). PubMed: 30387833
- Zhang J et al. Circular RNA_LARP4 inhibits cell proliferation and invasion of gastric cancer by sponging miR-424-5p and regulating LATS1 expression. Mol Cancer 16:151 (2017). PubMed: 28893265
- Xu M et al. Reversal effect of adenovirus-mediated human interleukin 24 transfection on the cisplatin resistance of A549/DDP lung cancer cells. Oncol Rep 38:2843-2851 (2017). PubMed: 29048638