Overview

  • Product name

  • Description

    Rabbit polyclonal to GAPDS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human GAPDS aa 161-391.

  • Positive control

    • NT2D1, IMR32, U87-MG, MCF7 and NIH3T3 cell lysates; MCF7 cells.
  • General notes

     This product was previously labelled as GAPDHS

     

Properties

Applications

Our Abpromise guarantee covers the use of ab153802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility.
  • Tissue specificity

    Testis specific.
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • Sequence similarities

    Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • Domain

    The testis-specific N-terminal extension mediates tight association with the cytoskeletal fibrous sheath of the spermatozoa flagellum, possibly via interchain disulfide-bonding of Cys-21 with sheath components.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • G3PT_HUMAN antibody
    • GAPD2 antibody
    • GAPDH 2 antibody
    • GAPDH-2 antibody
    • GAPDH2 antibody
    • GAPDHS antibody
    • GAPDS antibody
    • Glyceraldehyde 3 phosphate dehydrogenase spermatogenic antibody
    • Glyceraldehyde 3 phosphate dehydrogenase, testis specific antibody
    • Glyceraldehyde-3-phosphate dehydrogenase antibody
    • HSD 35 antibody
    • HSD35 antibody
    • Spermatogenic cell specific glyceraldehyde 3 phosphate dehydrogenase 2 antibody
    • Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2 antibody
    • Spermatogenic glyceraldehyde 3 phosphate dehydrogenase antibody
    • Spermatogenic glyceraldehyde-3-phosphate dehydrogenase antibody
    • testis-specific antibody
    see all

Images

  • Anti-GAPDS antibody (ab153802) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size: 45 kDa



    10% SDS PAGE
  • Anti-GAPDS antibody (ab153802) at 1/5000 dilution + NIH3T3 whole cell lysate at 30 µg

    Predicted band size: 45 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed MCF7 cells labeling GAPDS with ab153802 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

References

This product has been referenced in:

  • Liu X  et al. Aberrant expression of sperm-specific glycolytic enzymes are associated with poor sperm quality. Mol Med Rep 19:2471-2478 (2019). Read more (PubMed: 30720094) »
  • Yan L  et al. IL-21R functions as an oncogenic factor and is regulated by the lncRNA MALAT1/miR-125a-3p axis in gastric cancer. Int J Oncol 54:7-16 (2019). Read more (PubMed: 30387833) »
See all 4 Publications for this product

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