Key features and details
- Rabbit polyclonal to GAPex 5
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GAPex 5 antibody
DescriptionRabbit polyclonal to GAPex 5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Dog, Turkey, Pig, Xenopus laevis, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Platypus
A synthetic peptide corresponding to a region between residue 550 and 600 of human GAPex 5 (BAG10470.1).
- HeLa whole cell lysate (ab150035)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab86033 was affinity purified using an epitope specific to GAPex 5 immobilized on solid support
Our Abpromise guarantee covers the use of ab86033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Predicted molecular weight: 165 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceGAPex 5 plays a role in several processes such as endocytosis, insulin receptor internalisation and LC2A4/GLUT4 trafficking. It acts as both a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF). GAPex 5 is a GEF for the small G protein Rab31, promoting the exchange of GDP bound to Rab31 for GTP and thus regulating LC2A4/GLUT4 trafficking. In the absence of insulin, GAPex 5 maintains Rab31 in an active (GTP-bound) state, thereby promoting a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, keeping LC2A4/GLUT4 inside the cells. Following insulin stimulation, GAPex 5 is translocated to the plasma membrane, enabling the release of LC2A4/GLUT4 from the intracellular storage vesicles. GAPex 5 is also involved in EGFR trafficking and degradation, and also has GEF activity for Rab5 and GAP activity for Ras.
Cellular localizationCell Membrane (peripheral membrane protein) and Cytoplasmic
- GAPEX5 antibody
- GAPVD1 antibody
- GTPase activating protein and VPS9 domains 1 antibody
All lanes : Anti-GAPex 5 antibody (ab86033) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 165 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 71 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
ab86033, at 0.1 µg/ml, staining GAPex 5 in HeLa whole cell lysate immunoprecipitated using ab86033 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: by chemiluminescence with an exposure time of 30 seconds.
ab86033 has not yet been referenced specifically in any publications.