Recombinant
RabMAb

Recombinant Anti-GARNL1 antibody [EPR13592-38] (ab182570)

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Overview

  • Product name

    Anti-GARNL1 antibody [EPR13592-38]
    See all GARNL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13592-38] to GARNL1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human GARNL1 aa 700-800. The exact sequence is proprietary.
    Database link: Q6GYQ0

  • Positive control

    • 293 and HeLa cell lysate; Human kidney tissue; HeLa cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab182570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/100000. Detects a band of approximately 280 kDa (predicted molecular weight: 230 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Relevance

    GTPase activating Rap/RanGAP domain-like 1 (GARNL1) is an interacting partner of the transcription factor TCF3/isoform E12, mainly in the developing embryonic forebrain. It may be an important transcriptional regulator of downstream target genes under the control of TCF3/E12, by disrupting HLH dimer formation of TCF3/E12 with other proteins and may be involved in neuronal differentiation.

  • Cellular localization

    Cytoplasmic and Nuclear. Translocated to the nucleus when associated with TCF3/E12

  • Database links

    see all

  • Alternative names

    • DKFZp566D133 antibody
    • DKFZp667F074 antibody
    • GAP related interacting partner to E12 antibody
    • GAP related interacting protein to E12 antibody
    • GARN L1 antibody
    • GARNL 1 antibody
    • GRIPE antibody
    • GTPase activating RANGAP domain like 1 antibody
    • GTPase activating Rap/RanGAP domain like 1 antibody
    • KIAA0884 antibody
    • p240 antibody
    • Ral GTPase activating protein catalytic alpha subunit 1 antibody
    • Ral GTPase activating protein subunit alpha 1 antibody
    • Ral GTPase activating protein, alpha subunit 1 (catalytic) antibody
    • RalGAPalpha1 antibody
    • Tuberin like protein 1 antibody
    • TULIP 1 antibody
    • TULIP1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-GARNL1 antibody [EPR13592-38] (ab182570)
    Immunocytochemistry/ Immunofluorescence - Anti-GARNL1 antibody [EPR13592-38] (ab182570)

    Immunofluorescence analysis of HeLa cells labeling GARNL1 using ab182570 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with -20℃ Acetone. Counterstain: DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARNL1 antibody [EPR13592-38] (ab182570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARNL1 antibody [EPR13592-38] (ab182570)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GARNL1 using ab182570 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG was used as a secondary. Counterstain: Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-GARNL1 antibody [EPR13592-38] (ab182570)
    Western blot - Anti-GARNL1 antibody [EPR13592-38] (ab182570)
    Anti-GARNL1 antibody [EPR13592-38] (ab182570) at 1/100000 dilution + HeLa cell lysate at 1/10 dilution

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 230 kDa

  • Western blot - Anti-GARNL1 antibody [EPR13592-38] (ab182570)
    Western blot - Anti-GARNL1 antibody [EPR13592-38] (ab182570)
    Anti-GARNL1 antibody [EPR13592-38] (ab182570) at 1/100000 dilution + 293 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 230 kDa

References

ab182570 has not yet been referenced specifically in any publications.

Publishing research using ab182570? Please let us know so that we can cite the reference in this datasheet.

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