RecombinantRabMAb

Recombinant Anti-GARNL1 antibody [EPR13592-38] (ab182570)

Submit a review Submit a question

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13592-38] to GARNL1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GARNL1 antibody [EPR13592-38]
    See all GARNL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13592-38] to GARNL1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human GARNL1 aa 700-800. The exact sequence is proprietary.
    Database link: Q6GYQ0

  • Positive control

    • 293 and HeLa cell lysate; Human kidney tissue; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab182570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/100000. Detects a band of approximately 280 kDa (predicted molecular weight: 230 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Relevance

    GTPase activating Rap/RanGAP domain-like 1 (GARNL1) is an interacting partner of the transcription factor TCF3/isoform E12, mainly in the developing embryonic forebrain. It may be an important transcriptional regulator of downstream target genes under the control of TCF3/E12, by disrupting HLH dimer formation of TCF3/E12 with other proteins and may be involved in neuronal differentiation.

  • Cellular localization

    Cytoplasmic and Nuclear. Translocated to the nucleus when associated with TCF3/E12

  • Database links

    see all

  • Alternative names

    • DKFZp566D133 antibody
    • DKFZp667F074 antibody
    • GAP related interacting partner to E12 antibody
    • GAP related interacting protein to E12 antibody
    • GARN L1 antibody
    • GARNL 1 antibody
    • GRIPE antibody
    • GTPase activating RANGAP domain like 1 antibody
    • GTPase activating Rap/RanGAP domain like 1 antibody
    • KIAA0884 antibody
    • p240 antibody
    • Ral GTPase activating protein catalytic alpha subunit 1 antibody
    • Ral GTPase activating protein subunit alpha 1 antibody
    • Ral GTPase activating protein, alpha subunit 1 (catalytic) antibody
    • RalGAPalpha1 antibody
    • Tuberin like protein 1 antibody
    • TULIP 1 antibody
    • TULIP1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-GARNL1 antibody [EPR13592-38] (ab182570)
    Immunocytochemistry/ Immunofluorescence - Anti-GARNL1 antibody [EPR13592-38] (ab182570)

    Immunofluorescence analysis of HeLa cells labeling GARNL1 using ab182570 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with -20℃ Acetone. Counterstain: DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARNL1 antibody [EPR13592-38] (ab182570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARNL1 antibody [EPR13592-38] (ab182570)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GARNL1 using ab182570 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG was used as a secondary. Counterstain: Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-GARNL1 antibody [EPR13592-38] (ab182570)
    Western blot - Anti-GARNL1 antibody [EPR13592-38] (ab182570)
    Anti-GARNL1 antibody [EPR13592-38] (ab182570) at 1/100000 dilution + HeLa cell lysate at 1/10 dilution

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 230 kDa

  • Western blot - Anti-GARNL1 antibody [EPR13592-38] (ab182570)
    Western blot - Anti-GARNL1 antibody [EPR13592-38] (ab182570)
    Anti-GARNL1 antibody [EPR13592-38] (ab182570) at 1/100000 dilution + 293 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 230 kDa

  • Anti-GARNL1 antibody [EPR13592-38] (ab182570)
    Anti-GARNL1 antibody [EPR13592-38] (ab182570)

References (0)

Publishing research using ab182570? Please let us know so that we can cite the reference in this datasheet.

ab182570 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab182570.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com