
Recombinant Anti-GARNL1 antibody [EPR13592-38] (ab182570)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-GARNL1 antibody [EPR13592-38]
See all GARNL1 primary antibodies -
Description
Rabbit monoclonal [EPR13592-38] to GARNL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human GARNL1 aa 700-800. The exact sequence is proprietary.
Database link: Q6GYQ0 -
Positive control
- 293 and HeLa cell lysate; Human kidney tissue; HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13592-38 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab182570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/10000 - 1/100000. Detects a band of approximately 280 kDa (predicted molecular weight: 230 kDa). | |
IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
ICC/IF | 1/100. |
Target
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Relevance
GTPase activating Rap/RanGAP domain-like 1 (GARNL1) is an interacting partner of the transcription factor TCF3/isoform E12, mainly in the developing embryonic forebrain. It may be an important transcriptional regulator of downstream target genes under the control of TCF3/E12, by disrupting HLH dimer formation of TCF3/E12 with other proteins and may be involved in neuronal differentiation. -
Cellular localization
Cytoplasmic and Nuclear. Translocated to the nucleus when associated with TCF3/E12 -
Database links
- Entrez Gene: 253959 Human
- Entrez Gene: 56784 Mouse
- Entrez Gene: 56785 Rat
- Omim: 608884 Human
- SwissProt: Q6GYQ0 Human
- SwissProt: Q6GYP7 Mouse
- SwissProt: O55007 Rat
- Unigene: 113150 Human
see all -
Alternative names
- DKFZp566D133 antibody
- DKFZp667F074 antibody
- GAP related interacting partner to E12 antibody
see all
Images
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Immunofluorescence analysis of HeLa cells labeling GARNL1 using ab182570 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with -20℃ Acetone. Counterstain: DAPI.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARNL1 antibody [EPR13592-38] (ab182570)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GARNL1 using ab182570 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG was used as a secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Anti-GARNL1 antibody [EPR13592-38] (ab182570) at 1/100000 dilution + HeLa cell lysate at 1/10 dilution
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 230 kDa -
Anti-GARNL1 antibody [EPR13592-38] (ab182570) at 1/100000 dilution + 293 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 230 kDa
Protocols
Datasheets and documents
References
ab182570 has not yet been referenced specifically in any publications.