Recombinant Anti-GARNL1 antibody [EPR13592] (ab182148)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13592] to GARNL1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GARNL1 antibody [EPR13592]
See all GARNL1 primary antibodies -
Description
Rabbit monoclonal [EPR13592] to GARNL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293 and HeLa whole cell lysate (ab150035); 293 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13592 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 230 kDa).
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ICC/IF |
1/100.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 230 kDa). |
ICC/IF
1/100. |
Target
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Relevance
GTPase activating Rap/RanGAP domain-like 1 (GARNL1) is an interacting partner of the transcription factor TCF3/isoform E12, mainly in the developing embryonic forebrain. It may be an important transcriptional regulator of downstream target genes under the control of TCF3/E12, by disrupting HLH dimer formation of TCF3/E12 with other proteins and may be involved in neuronal differentiation. -
Cellular localization
Cytoplasmic and Nuclear. Translocated to the nucleus when associated with TCF3/E12 -
Database links
- Entrez Gene: 253959 Human
- Omim: 608884 Human
- SwissProt: Q6GYQ0 Human
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Alternative names
- DKFZp566D133 antibody
- DKFZp667F074 antibody
- GAP related interacting partner to E12 antibody
see all
Images
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Anti-GARNL1 antibody [EPR13592] (ab182148) at 1/10000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 230 kDa -
Immunofluorescent analysis of 293 cells (paraformaldehyde-fixed, 4%) labeling GARNL1 with ab182148 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 diution and counterstained with DAPI.
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Anti-GARNL1 antibody [EPR13592] (ab182148) at 1/10000 dilution + 293 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 230 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab182148 has not yet been referenced specifically in any publications.