Anti-GARS antibody (ab196496)
Key features and details
- Rabbit polyclonal to GARS
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-GARS antibody
See all GARS primary antibodies -
Description
Rabbit polyclonal to GARS -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human GARS (internal sequence). The exact sequence is proprietary.
Database link: P41250 -
Positive control
- HT29 whole cell lysate (ab3952) can be used as a positive control in WB. Jurkat, 293T, U87, Raji, M21, 22RV-1 cell lysates; mouse brain and spinal cord tissue lysates. MCF7 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/50 - 1/200.
|
|
WB |
1/500 - 1/2000. Predicted molecular weight: 83 kDa.
|
|
IHC-P |
1/50 - 1/200.
|
Notes |
---|
ICC/IF
1/50 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 83 kDa. |
IHC-P
1/50 - 1/200. |
Target
-
Relevance
GARS synthetase catalyzes the ligation of glycine with glycyl-tRNA using ATP and its mutation induces Charcot-Marie-Tooth (CMT) disease. -
Cellular localization
Cytoplasm. Mitochondrion. -
Database links
- Entrez Gene: 2617 Human
- Entrez Gene: 353172 Mouse
- Entrez Gene: 297113 Rat
- Omim: 600287 Human
- SwissProt: P41250 Human
- SwissProt: Q9CZD3 Mouse
- SwissProt: Q5I0G4 Rat
-
Alternative names
- AP 4 A synthetase antibody
- Charcot Marie Tooth neuropathy 2D antibody
- Charcot Marie Tooth neuropathy neuronal type D antibody
see all
Images
-
All lanes : Anti-GARS antibody (ab196496) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : U87 cell lysate
Lane 4 : Raji cell lysate
Lane 5 : M21 cell lysate
Lane 6 : 22RV-1 cell lysate
Lane 7 : HT29 cell lysate
Lane 8 : mouse brain tissue lysate
Lane 9 : mouse spinal cord tissue lysate
Predicted band size: 83 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat heart tissue labelling GARS with ab196496 at a dilution of 1/100.
-
Immunofluorescent analysis of MCF7 cells labeling GARS using ab196496 at 1/50 dilution. Blue: DAPI for nuclear staining.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab196496 has not yet been referenced specifically in any publications.