Product nameAnti-GARS antibody
See all GARS primary antibodies
DescriptionRabbit polyclonal to GARS
SpecificityThis antibody reacts specifically with 79kDa GARS protein.
Tested applicationsSuitable for: ICC/IF, IP, WB, IHC-FoFrmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein (Human)
- HEK293 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: Whole serum
Our Abpromise guarantee covers the use of ab42905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/3000 - 1/10000.|
|IP||1/3000 - 1/10000.|
|WB||1/5000. Predicted molecular weight: 83 kDa.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 22144914|
RelevanceGARS synthetase catalyzes the ligation of glycine with glycyl-tRNA using ATP and its mutation induces Charcot-Marie-Tooth (CMT) disease.
Cellular localizationCytoplasm. Mitochondrion.
- AP 4 A synthetase antibody
- Charcot Marie Tooth neuropathy 2D antibody
- Charcot Marie Tooth neuropathy neuronal type D antibody
Anti-GARS antibody (ab42905) at 1/5000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml
Goat anti-rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 83 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
ICC/IF image of ab42905 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42905, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab42905 staining GARS in murine sciatic nerve tissue by Immunohistochemistry (PFA perfusion fixed frozen sections).
Tissue was fixed with paraformaldehyde and blocked with 5% goat serum in PBS with 0.5% Triton X-100 for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in diluent) overnight at 4°C. An AlexaFluor®555-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
This product has been referenced in:
- Boulos S et al. Serine 207 phosphorylated lysyl-tRNA synthetase predicts disease-free survival of non-small-cell lung carcinoma. Oncotarget 8:65186-65198 (2017). Read more (PubMed: 29029422) »
- Malissovas N et al. Dimerization is required for GARS-mediated neurotoxicity in dominant CMT disease. Hum Mol Genet 25:1528-42 (2016). Read more (PubMed: 27008886) »