Overview

  • Product name

  • Description

    Mouse polyclonal to GARS
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length human GARS protein (ENSP00000373918; or amino acids 55-739 of Swissprot id P41250).

  • Positive control

    • Human placenta tissue lysate; GARS transfected 293T cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 75 kDa.

Target

  • Relevance

    GARS synthetase catalyzes the ligation of glycine with glycyl-tRNA using ATP and its mutation induces Charcot-Marie-Tooth (CMT) disease.
  • Cellular localization

    Cytoplasm. Mitochondrion.
  • Database links

  • Alternative names

    • AP 4 A synthetase antibody
    • Charcot Marie Tooth neuropathy 2D antibody
    • Charcot Marie Tooth neuropathy neuronal type D antibody
    • CMT2D antibody
    • Diadenosine tetraphosphate synthetase antibody
    • DSMAV antibody
    • EC 6.1.1.14 antibody
    • Glycine tRNA ligase antibody
    • Glycyl tRNA synthetase antibody
    • GlyRS antibody
    • HMN5 antibody
    • SMAD1 antibody
    see all

Images

  • Anti-GARS antibody (ab89522) at 1 µg/ml + human placenta tissue lysate at 50 µg

    Predicted band size: 75 kDa
    Observed band size: ~75 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-GARS antibody (ab89522) at 1 µg/ml

    Lane 1 : GARS transfected 293T cell lysate
    Lane 2 : Non transfected lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 75 kDa
    Observed band size: 75 kDa

References

This product has been referenced in:

  • Yang Y  et al. Clinical Features and Cytokine Profile in Myositis Patients with Anti-EJ Autoantibodies Detected by a Novel Immunoprecipitation Assay. Biomed Res Int 2019:1856180 (2019). Read more (PubMed: 31019965) »
  • Nafisinia M  et al. Compound heterozygous mutations in glycyl-tRNA synthetase (GARS) cause mitochondrial respiratory chain dysfunction. PLoS One 12:e0178125 (2017). WB ; Human . Read more (PubMed: 28594869) »
See all 2 Publications for this product

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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