Recombinant Anti-GARS antibody [AT4E10] (ab282154)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [AT4E10] to GARS
- Suitable for: WB, ICC
- Reacts with: Human
Overview
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Product name
Anti-GARS antibody [AT4E10]
See all GARS primary antibodies -
Description
Mouse monoclonal [AT4E10] to GARS -
Host species
Mouse -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human GARS aa 1-300.
Database link: P41250 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, U87MG, A431 cell lysate. ICC: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 0.75% Sodium chloride, 0.02% Potassium chloride, 0.13% Dibasic monohydrogen sodium phosphate, 0.02% Monobasic dihydrogen potassium phosphate, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AT4E10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab282154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 83 kDa.
|
|
| ICC |
1/100.
|
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 83 kDa. |
|
ICC
1/100. |
Target
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Relevance
GARS synthetase catalyzes the ligation of glycine with glycyl-tRNA using ATP and its mutation induces Charcot-Marie-Tooth (CMT) disease. -
Cellular localization
Cytoplasm. Mitochondrion. -
Database links
- Entrez Gene: 2617 Human
- Omim: 600287 Human
- SwissProt: P41250 Human
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Alternative names
- AP 4 A synthetase antibody
- Charcot Marie Tooth neuropathy 2D antibody
- Charcot Marie Tooth neuropathy neuronal type D antibody
see all
Images
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Western blot - Anti-GARS antibody (ab282154)All lanes : Anti-GARS antibody [AT4E10] (ab282154) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 5 : A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti-Mouse secondary antibody HRP-conjugated
Predicted band size: 83 kDaSDS-PAGE gel was transferred to PVDF membrane.
ECL detection system.
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Immunocytochemistry - Anti-GARS antibody (ab282154)Immunocytochemical analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling GARS with ab282154 at 1/100 dilution followed by Alexa Fluor 488 secondary antibody (green). DAPI was stained the cell nucleus (blue). -
![Anti-GARS antibody [AT4E10] (ab282154)](https://www.abcam.com/ps/products/282/ab282154/Images/ab282154-464269-benefitsofrecombinantantibodies.jpg)
Anti-GARS antibody [AT4E10] (ab282154)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab282154 has not yet been referenced specifically in any publications.
