Key features and details
- Rabbit polyclonal to GARS - C-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-GARS antibody - C-terminal
See all GARS primary antibodies
DescriptionRabbit polyclonal to GARS - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey
Synthetic peptide within Human GARS aa 689-739 (C terminal). The exact sequence is proprietary. NP_002038.2
Database link: P41250
- IP: HEK-293T whole cell lysate. WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226209 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 83 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceGARS synthetase catalyzes the ligation of glycine with glycyl-tRNA using ATP and its mutation induces Charcot-Marie-Tooth (CMT) disease.
Cellular localizationCytoplasm. Mitochondrion.
- AP 4 A synthetase antibody
- Charcot Marie Tooth neuropathy 2D antibody
- Charcot Marie Tooth neuropathy neuronal type D antibody
All lanes : Anti-GARS antibody - C-terminal (ab226209) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 83 kDa
Exposure time: 10 seconds
GARS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226209 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226209 at 0.4 µg/ml.
Lane 1: ab226209 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226209 has not yet been referenced specifically in any publications.