Product nameAnti-GARS antibody [EPR7157]
See all GARS primary antibodies
DescriptionRabbit monoclonal [EPR7157] to GARS
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GARS aa 700-800 (C terminal). The exact sequence is proprietary.
- Jurkat, HeLa, U87-MG, 293T, C6, RAW 264.7, and Neuro-2a cell lysates; Human colon tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab125008 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 83 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
RelevanceGARS synthetase catalyzes the ligation of glycine with glycyl-tRNA using ATP and its mutation induces Charcot-Marie-Tooth (CMT) disease.
Cellular localizationCytoplasm. Mitochondrion.
- AP 4 A synthetase antibody
- Charcot Marie Tooth neuropathy 2D antibody
- Charcot Marie Tooth neuropathy neuronal type D antibody
All lanes : Anti-GARS antibody [EPR7157] (ab125008) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Hela cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : 293T cell lysate
Lane 5 : C6 cell lysate
Lane 6 : RAW 264.7 cell lysate
Lane 7 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 83 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
ab125008 at 1/50 staining GARS in paraffin embedded Human colon tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab125008 has not yet been referenced specifically in any publications.