Anti-GART antibody [EPR11622] (ab169550)
- Datasheet
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Overview
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Product nameAnti-GART antibody [EPR11622]
See all GART primary antibodies -
DescriptionRabbit monoclonal [EPR11622] to GART
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment corresponding to residues in Human GART (UniProt P22102).
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Positive control
- HeLa, HepG2, K562 and A431 cell lysates; Human colonic adenocarcinoma and tonsil tissues; HepG2 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR11622
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab169550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/500. | |
| WB | 1/1000 - 1/5000. Predicted molecular weight: 107 kDa. | |
| IHC-P | 1/50 - 1/100. | |
| IP | 1/10 - 1/100. |
Target
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PathwayPurine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.
Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.
Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1. -
Sequence similaritiesIn the N-terminal section; belongs to the GARS family.
In the central section; belongs to the AIR synthase family.
In the C-terminal section; belongs to the GART family.
Contains 1 ATP-grasp domain. - Information by UniProt
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Database links
- Entrez Gene: 2618 Human
- Entrez Gene: 14450 Mouse
- Entrez Gene: 288259 Rat
- Omim: 138440 Human
- SwissProt: P22102 Human
- SwissProt: Q64737 Mouse
- Unigene: 473648 Human
- Unigene: 10281 Mouse
see all -
Alternative names
- 5'-phosphoribosylglycinamide transformylase antibody
- AIR synthase antibody
- AIRS antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)](https://www.abcam.com/ps/products/169/ab169550/Images/ab169550-217208-ab169550iccif.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)This image is courtesy of an Abreview submitted by Kirk McManusab169550 staining GART in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.
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![Western blot - Anti-GART antibody [EPR11622] (ab169550)](https://www.abcam.com/ps/products/169/ab169550/Images/GART-Primary-antibodies-ab169550-1.jpg)
Western blot - Anti-GART antibody [EPR11622] (ab169550)All lanes : Anti-GART antibody [EPR11622] (ab169550) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 107 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] (ab169550)](https://www.abcam.com/ps/products/169/ab169550/Images/GART-Primary-antibodies-ab169550-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] (ab169550)Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling GART with ab169550 at 1/50 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] (ab169550)](https://www.abcam.com/ps/products/169/ab169550/Images/GART-Primary-antibodies-ab169550-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] (ab169550)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling GART with ab169550 at 1/50 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)](https://www.abcam.com/ps/products/169/ab169550/Images/GART-Primary-antibodies-ab169550-5.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)Immunofluorescent analysis of HepG2 cells labeling GART with ab169550 at 1/100 dilution. -
![Western blot - Anti-GART antibody [EPR11622] (ab169550)](https://www.abcam.com/ps/products/169/ab169550/Images/GART-Primary-antibodies-ab169550-6.jpg)
Western blot - Anti-GART antibody [EPR11622] (ab169550)Anti-GART antibody [EPR11622] (ab169550) at 1/1000 dilution + immunoprecipitation pellet from HeLa cell lysate
Secondary
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG
Predicted band size: 107 kDa
Protocols
Datasheets and documents
References
ab169550 has not yet been referenced specifically in any publications.
