Recombinant Anti-GART antibody [EPR11622] (ab169550)
![Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)](https://www.abcam.com/ps/products/169/ab169550/Images/ab169550-217208-anti-gart-antibody-epr11622-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11622] to GART
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GART antibody [EPR11622]
See all GART primary antibodies -
Description
Rabbit monoclonal [EPR11622] to GART -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human GART.
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Positive control
- HeLa, HepG2, K562 and A431 cell lysates; Human colonic adenocarcinoma and tonsil tissues; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11622 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab169550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/500. | |
| WB | 1/1000 - 1/5000. Predicted molecular weight: 107 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Pathway
Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.
Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.
Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1. -
Sequence similarities
In the N-terminal section; belongs to the GARS family.
In the central section; belongs to the AIR synthase family.
In the C-terminal section; belongs to the GART family.
Contains 1 ATP-grasp domain. - Information by UniProt
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Database links
- Entrez Gene: 2618 Human
- Entrez Gene: 14450 Mouse
- Entrez Gene: 288259 Rat
- Omim: 138440 Human
- SwissProt: P22102 Human
- SwissProt: Q64737 Mouse
- Unigene: 473648 Human
- Unigene: 10281 Mouse
see all -
Alternative names
- 5'-phosphoribosylglycinamide transformylase antibody
- AIR synthase antibody
- AIRS antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)This image is courtesy of an Abreview submitted by Kirk McManus
ab169550 staining GART in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.
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![Western blot - Anti-GART antibody [EPR11622] (ab169550)](https://www.abcam.com/ps/products/169/ab169550/Images/ab169550-168211-anti-gart-antibody-epr11622-western-blot.jpg)
Western blot - Anti-GART antibody [EPR11622] (ab169550)All lanes : Anti-GART antibody [EPR11622] (ab169550) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 107 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] (ab169550)](https://www.abcam.com/ps/products/169/ab169550/Images/ab169550-168210-anti-gart-antibody-epr11622-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] (ab169550)Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling GART with ab169550 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] (ab169550)](https://www.abcam.com/ps/products/169/ab169550/Images/ab169550-168209-anti-gart-antibody-epr11622-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] (ab169550)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling GART with ab169550 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)](https://www.abcam.com/ps/products/169/ab169550/Images/ab169550-168208-anti-gart-antibody-epr11622-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)Immunofluorescent analysis of HepG2 cells labeling GART with ab169550 at 1/100 dilution. -
![Western blot - Anti-GART antibody [EPR11622] (ab169550)](https://www.abcam.com/ps/products/169/ab169550/Images/ab169550-168207-anti-gart-antibody-epr11622-western-blot.jpg)
Western blot - Anti-GART antibody [EPR11622] (ab169550)Anti-GART antibody [EPR11622] (ab169550) at 1/1000 dilution + immunoprecipitation pellet from HeLa cell lysate
Secondary
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG
Predicted band size: 107 kDa -
![Anti-GART antibody [EPR11622] (ab169550)](https://www.abcam.com/ps/products/169/ab169550/Images/ab169550-7-benefits-of-recombinant-antibodies.png)
Anti-GART antibody [EPR11622] (ab169550)
Protocols
Datasheets and documents
References (2)
ab169550 has been referenced in 2 publications.
- Thompson LL et al. Reduced SKP1 Expression Induces Chromosome Instability through Aberrant Cyclin E1 Protein Turnover. Cancers (Basel) 12:N/A (2020). PubMed: 32106628
- Song W et al. Edoxaban improves venous thrombosis via increasing hydrogen sulfide and homocysteine in rat model. Mol Med Rep 16:7706-7714 (2017). PubMed: 28944880
