Recombinant
RabMAb

Recombinant Anti-GART antibody [EPR11622] (ab169550)

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Overview

  • Product name

    Anti-GART antibody [EPR11622]
    See all GART primary antibodies

  • Description

    Rabbit monoclonal [EPR11622] to GART

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment corresponding to Human GART.

  • Positive control

    • HeLa, HepG2, K562 and A431 cell lysates; Human colonic adenocarcinoma and tonsil tissues; HepG2 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
WB 1/1000 - 1/5000. Predicted molecular weight: 107 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Pathway

      Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.
      Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.
      Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.

    • Sequence similarities

      In the N-terminal section; belongs to the GARS family.
      In the central section; belongs to the AIR synthase family.
      In the C-terminal section; belongs to the GART family.
      Contains 1 ATP-grasp domain.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 5'-phosphoribosylglycinamide transformylase antibody
      • AIR synthase antibody
      • AIRS antibody
      • GAR transformylase antibody
      • GARS antibody
      • GART antibody
      • GARTF antibody
      • Glycinamide ribonucleotide synthetase antibody
      • MGC47764 antibody
      • PAIS antibody
      • PGFT antibody
      • Phosphoribosyl-aminoimidazole synthetase antibody
      • Phosphoribosylglycinamide formyltransferase antibody
      • Phosphoribosylglycinamide formyltransferase phosphoribosylglycinamide synthetase phosphoribosylaminoimidazole synthetase antibody
      • Phosphoribosylglycinamide formyltransferase, EC 2.1.2.29 antibody
      • Phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase antibody
      • Phosphoribosylglycinamide synthetase antibody
      • PRGS antibody
      • PUR2_HUMAN antibody
      • Trifunctional purine biosynthetic protein adenosine 3 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)
      Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)This image is courtesy of an Abreview submitted by Kirk McManus

      ab169550 staining GART in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.

      See Abreview

    • Western blot - Anti-GART antibody [EPR11622] (ab169550)
      Western blot - Anti-GART antibody [EPR11622] (ab169550)
      All lanes : Anti-GART antibody [EPR11622] (ab169550) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 107 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] (ab169550)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] (ab169550)

      Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling GART with ab169550 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] (ab169550)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody [EPR11622] (ab169550)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling GART with ab169550 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)
      Immunocytochemistry/ Immunofluorescence - Anti-GART antibody [EPR11622] (ab169550)
      Immunofluorescent analysis of HepG2 cells labeling GART with ab169550 at 1/100 dilution.
    • Western blot - Anti-GART antibody [EPR11622] (ab169550)
      Western blot - Anti-GART antibody [EPR11622] (ab169550)
      Anti-GART antibody [EPR11622] (ab169550) at 1/1000 dilution + immunoprecipitation pellet from HeLa cell lysate

      Secondary
      HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

      Predicted band size: 107 kDa

    References

    This product has been referenced in:

    • Song W  et al. Edoxaban improves venous thrombosis via increasing hydrogen sulfide and homocysteine in rat model. Mol Med Rep 16:7706-7714 (2017). Read more (PubMed: 28944880) »
    See 1 Publication for this product

    Publishing research using ab169550? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-GART antibody [EPR11622]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jan 23 2014

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