Key features and details
- Rabbit polyclonal to GART - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GART antibody - N-terminal
See all GART primary antibodies
DescriptionRabbit polyclonal to GART - N-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Cow
Recombinant fragment corresponding to a region within N-terminal amino acids 1-208 of Human GART (P22102).
- A431 and HeLa whole cell lysates, HeLa cells
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab153818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 107 kDa.|
|ICC/IF||1/100 - 1/1000.|
PathwayPurine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.
Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.
Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Sequence similaritiesIn the N-terminal section; belongs to the GARS family.
In the central section; belongs to the AIR synthase family.
In the C-terminal section; belongs to the GART family.
Contains 1 ATP-grasp domain.
- Information by UniProt
- 5'-phosphoribosylglycinamide transformylase antibody
- AIR synthase antibody
- AIRS antibody
All lanes : Anti-GART antibody - N-terminal (ab153818) at 1/5000 dilution
Lane 1 : A431 whole cell
Lane 2 : HeLa whole cell
Lysates/proteins at 30 µg per lane.
Predicted band size: 107 kDa
7.5% SDS PAGE
Immunofluorescent analysis of methanol-fixed HeLa cells labeling GART with ab153818 at 1/500 dilution. Image at the bottom costained with Hoechst 33342.
ab153818 has not yet been referenced specifically in any publications.